School of Public Health, and Key Laboratory of Environment and Human Health of Hebei Province, Hebei Medical University, Shijiazhuang, P. R. China.
Department of Nutrition, The First Hospital of Hebei Medical University, Shijiazhuang, P. R. China.
Electrophoresis. 2020 Oct;41(18-19):1584-1591. doi: 10.1002/elps.202000122. Epub 2020 Jul 30.
In the present research, field-amplified sample injection-CZE (FASI-CZE) coupled with a diode array detector was established to determine trace level sulfa antibiotic. Sulfathiazole, sulfadiazine, sulfamethazine, sulfadimethoxine, sulfamethoxazole, and sulfisoxazole were selected as analytes for the experiments. The background electrolyte solution consisted of 70.0 mmol/L borax and 60.0 mmol/L boric acid (including 10% methanol, pH 9.1). The plug was 2.5 mmol/L borax, which was injected into the capillary at a pressure of 0.5 psi for 5 s. Then the sample was injected into the capillary at an injection voltage of -10 kV for 20 s. The electrophoretic separation was carried out under a voltage of +19 kV. The capillary temperature was maintained at 20˚C throughout the analysis, and six sulfonamides were completely separated within 35 min. Compared with pressure injection-CZE, the sensitivity of FASI-CZE was increased by 6.25-10.0 times, and the LODs were reduced from 0.2-0.5 to 0.02-0.05 μg/mL. The method was applied to the determination of sulfonamides in river water and particulate matter samples. The recoveries were 78.59-106.59%. The intraday and interday precisions were 2.89-7.35% and 2.77-7.09%, respectively. This provides a simpler and faster method for the analysis of sulfa antibiotic residues in environmental samples.
在本研究中，建立了场放大样品进样- CZE（FASI-CZE）与二极管阵列检测器耦合，用于测定痕量水平的磺胺抗生素。选择磺胺噻唑、磺胺嘧啶、磺胺甲噁唑、磺胺二甲氧嘧啶、磺胺甲噁唑和磺胺异噁唑作为分析物进行实验。背景电解质溶液由 70.0 mmol/L 硼砂和 60.0 mmol/L 硼酸（含 10%甲醇，pH 9.1）组成。塞子为 2.5 mmol/L 硼砂，在 0.5 psi 的压力下注入毛细管 5 s。然后在 -10 kV 的进样电压下将样品注入毛细管 20 s。在 +19 kV 的电压下进行电泳分离。整个分析过程中保持毛细管温度在 20°C，6 种磺胺类药物在 35 分钟内完全分离。与压力进样-CZE 相比，FASI-CZE 的灵敏度提高了 6.25-10.0 倍，LOD 从 0.2-0.5 降低到 0.02-0.05 μg/mL。该方法应用于河水和颗粒物样品中磺胺类药物的测定。回收率为 78.59-106.59%。日内和日间精密度分别为 2.89-7.35%和 2.77-7.09%。这为环境样品中磺胺类抗生素残留的分析提供了一种更简单、更快的方法。
