Noorbakhsh Samileh, Farhadi Mohammad, Haghighi Faezeh, Minaeian Sara, Hasanabad Morteza Haghighi
Pediatric Infectious Diseases Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran.
Head and Neck Research Center, Iran University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2020 Jun;12(3):198-203.
Cytomegalovirus (CMV) constitutes the most common viral cause of congenital infections in newborns worldwide. There are a significant number of asymptomatic newborns with congenital CMV infection in Iran, which may develop long-term sequelae of infection. Unfortunately, limited data exsists from Iran on the rate of congenital CMV infection among neonates. The current study was aimed to investigate the prevalence of congenital CMV infection among Iranian neonates by testing Guthrie cards.
Guthrie cards were collected from infants within 2 weeks of life, and total DNA was extracted from samples by thermal shock and evaluated for CMV DNA using nested-PCR assay. CMV infection in newborns was confirmed through a commercial CMV PCR kit. Infected infants underwent further evaluation at the hospital.
CMV infection was identified in four of 1174 infants (0.34%) which is approximately 3 cases per 1000 live births. Infected infants were asymptomatic at birth and had a normal hearing status similar to other children. There were no factors in relation with CMV infection among newborns.
According to the results of this study, infected infants with congenital CMV infection could identify at early stage by testing Guthrie cards (within 21 days of life). Furthermore, since there is a lack of CMV knowledge in our population, educating and effective counseling by obstetricians/ gynecologists to the pregnant women are recommended.
巨细胞病毒（CMV）是全球新生儿先天性感染最常见的病毒病因。伊朗有大量先天性CMV感染的无症状新生儿，这些新生儿可能会出现感染的长期后遗症。遗憾的是，伊朗关于新生儿先天性CMV感染率的数据有限。本研究旨在通过检测格思里卡片来调查伊朗新生儿先天性CMV感染的患病率。
收集出生2周内婴儿的格思里卡片，通过热休克从样本中提取总DNA，并使用巢式PCR测定法评估CMV DNA。新生儿的CMV感染通过商用CMV PCR试剂盒进行确认。感染婴儿在医院接受进一步评估。
在1174名婴儿中有4名（0.34%）被确诊为CMV感染，即每1000例活产中约有3例。感染婴儿出生时无症状，听力状况与其他儿童相似。新生儿中没有与CMV感染相关的因素。
根据本研究结果，先天性CMV感染的婴儿可通过检测格思里卡片（出生21天内）在早期被识别出来。此外，由于我们的人群中缺乏CMV知识，建议产科医生/妇科医生对孕妇进行教育和有效的咨询。
