Saratov State University, Saratov, Russia.
Tomsk State University, Tomsk, Russia.
J Biophotonics. 2020 Nov;13(11):e201960249. doi: 10.1002/jbio.201960249. Epub 2020 Aug 26.
Skin optical clearing effect ex vivo and in vivo was achieved by topical application of low molecular weight paramagnetic magnetic resonance contrast agents. This novel feature has not been explored before. By using collimated transmittance the diffusion coefficients of three clinically used magnetic resonance contrast agents, that is Gadovist, Magnevist and Dotarem as well as X-ray contrast agent Visipaque in mouse skin were determined ex vivo as (4.29 ± 0.39) × 10 cm /s, (5.00 ± 0.72) × 10 cm /s, (3.72 ± 0.67) × 10 cm /s and (1.64 ± 0.18) × 10 cm /s, respectively. The application of gadobutrol (Gadovist) resulted in efficient optical clearing that in general, was superior to other contrast agents tested and allowed to achieve: (a) more than 12-fold increase of transmittance over 10 minutes after application ex vivo; (b) markedly improved images of skin architecture obtained with optical coherence tomography; (c) an increase of the fluorescence intensity/background ratio in TagRFP-red fluorescent marker protein expressing tumor by five times after 15 minutes application into the skin in vivo. The obtained results have immediate implications for multimodality imaging because many contrast agents are capable of simultaneously enhancing the contrast of multiple imaging modalities.
皮肤光学透明效果在体外用低分子量顺磁磁共振对比剂实现。这一新颖的特性以前没有被探索过。通过使用准直透射，我们确定了三种临床使用的磁共振对比剂，即 Gadovist、Magnevist 和 Dotarem 以及 X 射线对比剂 Visipaque 在小鼠皮肤中的扩散系数分别为（4.29±0.39）×10cm/s、（5.00±0.72）×10cm/s、（3.72±0.67）×10cm/s和（1.64±0.18）×10cm/s。Gadobutrol（Gadovist）的应用导致了有效的光学透明，通常优于其他测试的对比剂，并允许实现：（a）在体外用 10 分钟后透射率增加超过 12 倍；（b）明显改善光学相干断层扫描获得的皮肤结构图像；（c）在体内应用 15 分钟后，TagRFP 红色荧光标记蛋白表达肿瘤的荧光强度/背景比增加五倍。这些结果立即对多模态成像具有重要意义，因为许多对比剂能够同时增强多种成像模式的对比度。
