通过荧光显微镜观察过敏性紫癜期间的实时血管和IgA动态变化。
Real-time vascular and IgA dynamics during Henoch-Schönlein purpura by fluorescent microscopy.
作者信息
Zhu Yujie, Wang Shaoyang, Xu Hui, He Hao, Pan Meng
机构信息
Department of Dermatology, Rui Jin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Department of Dermatology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
出版信息
Biomed Opt Express. 2021 Nov 29;12(12):7826-7834. doi: 10.1364/BOE.442454. eCollection 2021 Dec 1.
Henoch-Schönlein purpura (HSP) is a typical cutaneous immune skin disease, usually diagnosed by invasive biopsy. In this study, we develop a noninvasive optical method by combining optical clearing, confocal microscopy and immune-staining together to present the real-time in vivo dynamics of blood vessels, IgA molecules, and T cells in a HSP rat model. The small vessels in the skin are found with acute damage and then hyperplasia, which enhances deposition of IgA complexes in blood vessels. The migrating T cells in blood vessels in HSP regions can be detected by setting fast line scanning in this method. Our method provides vascular, cellular, and molecular dynamics during HSP development and is thus of great potential in research and diagnosis of HSP and other skin diseases.
过敏性紫癜（HSP）是一种典型的皮肤免疫性疾病，通常通过侵入性活检进行诊断。在本研究中，我们将光学透明、共聚焦显微镜和免疫染色相结合，开发出一种非侵入性光学方法，以呈现过敏性紫癜大鼠模型中血管、免疫球蛋白A（IgA）分子和T细胞的实时体内动态变化。研究发现，皮肤中的小血管会先出现急性损伤，然后增生，这会增强IgA复合物在血管中的沉积。通过在该方法中设置快速线扫描，可以检测到过敏性紫癜区域血管中迁移的T细胞。我们的方法提供了过敏性紫癜发展过程中的血管、细胞和分子动态变化，因此在过敏性紫癜和其他皮肤疾病的研究及诊断中具有巨大潜力。
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