Subhash Narayanan, Mohanan Changatharayil N, Mallia Rupananda J, Muralidharan Vadekkeveetil
Centre for Earth Science Studies, PB 7250, Thuruvikkal PO, Trivandrum 695031, India.
Funct Plant Biol. 2004 Aug;31(7):709-719. doi: 10.1071/FP03253.
The effects of drought and petrol engine exhaust pollutants, such as SO and NO and suspended particulate matter (SPM), on the photosynthetic activity of colocasia [Colocasia esculenta (L.) Schott], kacholam (Kaempferia galanga L.) and tapioca (Manihot esculenta Crantz) plants were studied from in vivo laser-induced chlorophyll fluorescence (LICF) spectra. An open-top chamber (OTC) of 2.5 m diameter and 3 m height incorporating an air-filtering unit was developed for this study. Plants grown inside the OTC were exposed to exhaust emissions from a two-stroke Birla Yamaha genset for 10 d, while a control group was maintained outside. Gaseous pollutants and SPM present inside the OTC during the exposure period were measured with a high-volume air sampler. The steady-state LICF spectra of the control and treated plants were recorded in the 650-750-nm region. Fluorescence induction kinetics (Kautsky effect) was also recorded during the stress period from dark-adapted intact plant leaves at the chlorophyll bands of 685 and 730 nm. The vitality indexes (Rfd-685 and Rfd-730) and stress adaptation index (Ap) derived from the induction kinetics were utilised along with the chlorophyll fluorescence intensity ratio (F685 / F730) for evaluation of stress-induced changes in plants.
通过体内激光诱导叶绿素荧光（LICF）光谱，研究了干旱以及汽油发动机尾气污染物，如二氧化硫、一氧化氮和悬浮颗粒物（SPM），对芋头[Colocasia esculenta (L.) Schott]、山柰（Kaempferia galanga L.）和木薯（Manihot esculenta Crantz）植株光合活性的影响。为此研究开发了一个直径2.5米、高3米并配有空气过滤装置的开顶式气室（OTC）。在OTC内生长的植株暴露于一台二冲程Birla Yamaha发电机组的尾气排放中10天，而对照组植株则置于室外。在暴露期间，使用大容量空气采样器测量OTC内的气态污染物和SPM。在650 - 750纳米区域记录对照植株和处理后植株的稳态LICF光谱。在胁迫期间，还从暗适应的完整植株叶片在685纳米和730纳米叶绿素波段记录荧光诱导动力学（考茨基效应）。从诱导动力学得出的活力指数（Rfd - 685和Rfd - 730）和胁迫适应指数（Ap），与叶绿素荧光强度比（F685 / F730）一起用于评估胁迫诱导的植株变化。
