Lichtenthaler H K, Buschmann C, Rinderle U, Schmuck G
Radiat Environ Biophys. 1986;25(4):297-308. doi: 10.1007/BF01214643.
In vivo chlorophyll fluorescence measurements have become a valuable tool in ecophysiology. Fluorescence emission spectra are influenced by the reabsorption of the tissue and indicate the composition of the antenna system and are influenced by the chlorophyll content per leaf area. The fluorescence induction kinetics ("Kautsky effect") can be used to study photosynthetic activity. These rapid, non-destructive methods can be applied for ecophysiological field research to check the vitality of plants and to document stress effects on the photosynthetic apparatus. The Rfd-values (Rfd = fd/fs), the ratio of the fluorescence decrease (fd) to the steady state fluorescence (fs), can be taken as a rapid vitality index of the leaves and trees. We here describe fundamental chlorophyll fluorescence results of leaves which are needed for the interpretation of in vivo fluorescence signatures in stress physiology and in the forest dieback research.
体内叶绿素荧光测量已成为生态生理学中的一种重要工具。荧光发射光谱受组织重吸收的影响，可指示天线系统的组成，并受每叶面积叶绿素含量的影响。荧光诱导动力学（“考茨基效应”）可用于研究光合活性。这些快速、无损的方法可应用于生态生理学野外研究，以检查植物的活力，并记录对光合器官的胁迫效应。Rfd值（Rfd = fd/fs），即荧光下降（fd）与稳态荧光（fs）的比值，可作为叶片和树木的快速活力指标。我们在此描述叶片的基本叶绿素荧光结果，这些结果是解释胁迫生理学和森林衰退研究中体内荧光特征所必需的。
