Franzini C, Cattozzo G, Pagani A
Laboratorio di analisi, Ospedale Civile, Rho, Milano.
Quad Sclavo Diagn. 1988 Jan-Dec;24(1-4):1-8.
Serum bilirubin has been fractionated by means of a gel-filtration technique, on Bio-Gel P-10 columns and with caffeine solution as eluent. When sera containing high concentration of direct-reacting bilirubin were applied to the column, a bilirubin fraction was eluted with the protein. This bilirubin fraction is not set free from protein in the presence of urea, guanidine and sodium dodecyl sulfate; moreover it exhibits peculiar behaviour in the direct and indirect diazo-reaction and in enzymatic oxidation by bilirubin-oxidase as well. On the basis of these data, such bilirubin fraction has been identified with the previously described delta-bilirubin. A method for the quantitative measurement of delta-bilirubin, based on gel-filtration separation followed by diazo-reaction, has been worked out. Results by this method satisfactorily compared with the Kodak Ektachem method.
血清胆红素已通过凝胶过滤技术在Bio-Gel P-10柱上以咖啡因溶液作为洗脱剂进行了分级分离。当将含有高浓度直接反应胆红素的血清应用于柱时，一种胆红素级分与蛋白质一起被洗脱。在尿素、胍和十二烷基硫酸钠存在下，这种胆红素级分不会从蛋白质中释放出来；此外，它在直接和间接重氮反应以及胆红素氧化酶的酶促氧化中也表现出特殊行为。基于这些数据，这种胆红素级分已被鉴定为先前描述的δ-胆红素。已经制定了一种基于凝胶过滤分离然后重氮反应的δ-胆红素定量测量方法。该方法的结果与柯达Ektachem方法进行了令人满意的比较。
