Qualmann K, Rehage J, Scholz H
Clinic for Cattle, School of Veterinary Medicine, Hannover.
Dtsch Tierarztl Wochenschr. 1997 Sep;104(9):354-8.
Application of a HPLC-technique according to MURACA & BLANCKAERT (1983) for accurate determination of the relative amounts of unconjugated bilirubin and its sugar mono- and diconjugates in bovine plasma is described and the results are compared with those of the conventional diazo-method. The diazo-assay yielded values for total and unconjugated bilirubin which were considerably higher than those obtained by the HPLC-technique. This is probably due to unidentified diazo-positive compounds distinct from bilirubin. The direct-reacting fraction in the diazo-assay showed little or no agreement with the fraction of total ester conjugates determined by HPLC. Results indicate that the diagnostical value of bilirubin subfractioning by the diazo-method as a liver function test in cattle must be reconsidered.
描述了一种根据穆拉卡和布兰克尔特（1983年）的方法应用高效液相色谱技术准确测定牛血浆中未结合胆红素及其单糖和双糖共轭物相对含量的方法，并将结果与传统重氮法的结果进行了比较。重氮测定法得出的总胆红素和未结合胆红素的值明显高于高效液相色谱技术获得的值。这可能是由于存在与胆红素不同的未鉴定的重氮阳性化合物。重氮测定法中的直接反应部分与高效液相色谱法测定的总酯共轭物部分几乎没有一致性。结果表明，必须重新考虑重氮法对胆红素进行亚组分分析作为牛肝功能测试的诊断价值。
