Pasqualetti P, Colantonio D, Casale R, Colangeli S, Natali G
Dipartimento di medicina interna e sanità pubblica, Università, L'Aquila.
Quad Sclavo Diagn. 1988 Jan-Dec;24(1-4):89-95.
Circadian rhythm of lymphocyte subsets was investigated in four healthy subjects, males, aged 35-58 years old. After a period of ambiental synchronization, venous blood samples were taken during a span of a day at 0.00 a.m., 4.00 a.m., 8.00 a.m., noon, 4.00 p.m. and 8.00 p.m. Lymphocyte subsets (OKT3, OKT4, OKT8, OKB7, OKJa1) were determined by monoclonal antibodies method, and serum level of cortisol by radioimmunoassay method. The OKT4/OKT8 ratio was also calculated. Data were analyzed by chronograms (mean +/- 1SD) and by cosinor method. Results show a significant circadian rhythm for each lymphocyte subset and for serum cortisol levels. The lowest levels of all circulating subsets were seen between noon and 4.00 p.m. and the highest levels around midnight, inversely related with the circadian rhythm of serum cortisol. The OKT4/OKT8 ratio, on the contrary, was relatively constant during the day, without a significant circadian rhythm. These observations have laboratoristic, clinical, and therapeutic implications and should be considered in the course of immunological studies.
在4名年龄在35 - 58岁的健康男性受试者中研究了淋巴细胞亚群的昼夜节律。经过一段时间的环境同步后，于凌晨0:00、凌晨4:00、上午8:00、中午、下午4:00和晚上8:00在一天的时间段内采集静脉血样。采用单克隆抗体法测定淋巴细胞亚群（OKT3、OKT4、OKT8、OKB7、OKJa1），采用放射免疫分析法测定血清皮质醇水平。还计算了OKT4/OKT8比值。数据通过时序图（均值±1标准差）和余弦法进行分析。结果显示每个淋巴细胞亚群和血清皮质醇水平均有显著的昼夜节律。所有循环亚群的最低水平出现在中午至下午4:00之间，最高水平出现在午夜左右，与血清皮质醇的昼夜节律呈负相关。相反，OKT4/OKT8比值在一天中相对恒定，无显著的昼夜节律。这些观察结果具有实验室、临床和治疗意义，在免疫学研究过程中应予以考虑。
