Miyawaki T, Taga K, Nagaoki T, Seki H, Suzuki Y, Taniguchi N
Clin Exp Immunol. 1984 Mar;55(3):618-22.
The circadian variations in circulating T cell subsets defined by monoclonal antibodies in eight healthy male volunteers were evaluated in whole blood using a flow cytometry. In all subjects, the number of lymphocytes showed a clear rhythmicity with high values at night and low values during the day. This circadian variation in circulating lymphocytes appeared to reflect largely a change in the number of T cells rather than B cells. The percentage of OKT3+ and OKT11+ cells showed a similar fluctuation with a peak at night and a depression during the day. It was found that the percentage of OKT4+ cells varied in parallel with that of T cells, particularly of OKT3+ cells, but the OKT8+ subset was not appreciably altered over a 24 h period. Thus, a circadian variation of T cells could be largely accounted for by a circadian change of OKT4+ cells. Plasma cortisol levels showed an expected circadian variation. It was also shown that there might be an intimate relationship between these circadian changes of T cell subsets and plasma cortisol levels.
利用流式细胞术对8名健康男性志愿者全血中由单克隆抗体定义的循环T细胞亚群的昼夜变化进行了评估。在所有受试者中，淋巴细胞数量呈现出明显的节律性，夜间值高，白天值低。循环淋巴细胞的这种昼夜变化似乎主要反映了T细胞数量的变化，而非B细胞。OKT3 +和OKT11 +细胞的百分比呈现出类似的波动，夜间达到峰值，白天则下降。研究发现，OKT4 +细胞的百分比与T细胞，特别是OKT3 +细胞的百分比平行变化，但OKT8 +亚群在24小时内没有明显变化。因此，T细胞的昼夜变化在很大程度上可能是由OKT4 +细胞的昼夜变化引起的。血浆皮质醇水平呈现出预期的昼夜变化。研究还表明，T细胞亚群的这些昼夜变化与血浆皮质醇水平之间可能存在密切关系。
