Kumar Anshuman, Kaiser Brent N, Siddiqi M Yaeesh, Glass Anthony D M
Department of Botany, University of British Columbia, 6270 University Blvd, Vancouver, Canada V6T 1Z4.
Funct Plant Biol. 2006 May;33(4):339-346. doi: 10.1071/FP05268.
In rice (Oryza sativa L.) OsAMT1.1 is the most active and / or most N-responsive gene responsible for high-affinity NH transport (HATS) activity. We measured NH influx and plant biomass in transgenic overexpression lines and two wild type cultivars of rice, Jarrah and Taipei, with one or more copies of OsAMT1.1. NH influx was higher for the overexpression lines of Jarrah line when grown at 10 µm external NH concentration, but not for the overexpression lines of Taipei. For seedlings grown at 2 mm external NH concentration Jarrah lines 77-1 and 75-4 showed an increased influx; however, two overexpression lines of Taipei showed reduced influx rates. The biomasses of the transgenic lines grown at low and high external NH concentrations were either reduced or showed no statistically significant differences compared with wild type lines. While NH influx into roots of Jarrah line 75-4 grown at 10 µm external NH concentration was significantly higher than in wild type, measurements of NH efflux revealed no differences, and thus net uptake of NH was higher in this overexpression line.
在水稻（Oryza sativa L.）中，OsAMT1.1是负责高亲和力NH转运（HATS）活性的最活跃和/或对氮响应最强的基因。我们测量了携带一个或多个OsAMT1.1拷贝的转基因过表达系以及两个野生型水稻品种Jarrah和台北的NH流入量和植株生物量。当在外部NH浓度为10µm的条件下生长时，Jarrah系的过表达系的NH流入量更高，但台北的过表达系并非如此。对于在外部NH浓度为2mm的条件下生长的幼苗，Jarrah系77 - 1和75 - 4的流入量增加；然而，台北的两个过表达系的流入速率降低。与野生型品系相比，在低和高外部NH浓度下生长的转基因系的生物量要么减少，要么没有统计学上的显著差异。虽然在外部NH浓度为10µm的条件下生长的Jarrah系75 - 4的根部NH流入量显著高于野生型，但NH流出量的测量结果没有差异，因此该过表达系的NH净吸收量更高。
