Zhang Chang, Luo Jia-Jun, Zuo Jing-Bo, Zhang Zheng, Wang Shi-Ting, Zhang Xiao-Jia, Fu Tian-Si, Feng Yu-Long
Liaoning Key Laboratory for Biological Invasions and Global Changes, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning, China.
Front Plant Sci. 2022 Oct 26;13:1035137. doi: 10.3389/fpls.2022.1035137. eCollection 2022.
Soil nitrogen forms are important for exotic plant invasions. However, little effort has been made to study the molecular mechanisms underlying the utilization of different N forms in co-occurring invasive and native plants. The invasive plant prefers nitrate relative to ammonium, and mainly invades nitrate-dominated environments, while it co-occurring native congener prefers ammonium. Here, we addressed the genetic bases for the interspecific difference in ammonium use and the effects of gibberellin (GA). Twenty-six transcripts related with GA biosynthesis and ammonium utilization were induced by ammonium in , while only ten in and none for ammonium uptake. , and may be crucial for the strong ability to absorb and assimilate ammonium in . All tested transcripts were significantly up-regulated by GA1 and GA4 in . , which was also induced by ammonium, may be involved in this regulation. Consistently, glutamine synthetase activity increased significantly with increasing ammonium-N/nitrate-N ratio for , while decreased for . Our study is the first to determine the molecular mechanisms with which invasive and native plants use ammonium differently, contributing to understanding the invasion mechanisms of and its invasion habitat selection.
土壤氮形态对外来植物入侵很重要。然而，对于同时存在的入侵植物和本地植物利用不同氮形态的分子机制，人们研究得很少。这种入侵植物相对于铵更偏好硝酸盐，主要入侵以硝酸盐为主的环境，而与其同时存在的本地同属植物则偏好铵。在这里，我们探讨了铵利用种间差异的遗传基础以及赤霉素（GA）的影响。26个与GA生物合成和铵利用相关的转录本在[具体植物1]中被铵诱导，而在[具体植物2]中只有10个，且没有与铵吸收相关的转录本。[基因1]、[基因2]和[基因3]可能对[具体植物1]吸收和同化铵的强大能力至关重要。在[具体植物1]中，所有测试的转录本都被GA1和GA4显著上调。[基因4]也被铵诱导，可能参与了这一调控。一致地，[具体植物i]的谷氨酰胺合成酶活性随着铵态氮/硝态氮比例的增加而显著增加，而[具体植物j]则降低。我们的研究首次确定了入侵植物和本地植物不同地利用铵的分子机制，有助于理解[具体植物名称]的入侵机制及其入侵生境选择。
