Coram Tristan E, Pang Edwin C K
RMIT University, School of Applied Sciences, Biotechnology and Environmental Biology, Building 223, Level 1, Plenty Road, Bundoora, Vic. 3083, Australia.
Funct Plant Biol. 2007 Feb;34(1):52-64. doi: 10.1071/FP06250.
Using microarray technology and a set of chickpea (Cicer arietinum L.) unigenes and grasspea (Lathyrus sativus L.) expressed sequence tags, chickpea responses to treatments with the defence signalling compounds salicylic acid (SA), methyl jasmonate (MeJA) and aminocyclopropane carboxylic acid (ACC) were studied in three chickpea genotypes with ranging levels of resistance to ascochyta blight [Ascochyta rabiei (Pass.) L.]. The experimental system minimised environmental effects and was conducted in reference design, where samples from untreated controls acted as references against post-treatment samples. Microarray observations were also validated by quantitative reverse transcription-polymerase chain reaction. The time-course expression patterns of 715 experimental microarray features resulted in differential expression of 425 transcripts. The A. rabiei resistant chickpea genotypes showed a more substantial range of defence-related gene induction by all treatments, indicating that they may possess stronger abilities to resist pathogens. Further, the involvement of SA, MeJA and ACC signalling was identified for the regulation of some important A. rabiei responsive transcripts, as well as cross-talk between these pathways. In the current study we also found evidence to suggest the involvement of A. rabiei-specific signalling mechanisms for the induction of several transcripts that were previously implicated in A. rabiei resistance. This study characterised the regulatory mechanisms of many chickpea transcripts that may be important in defence against various pathogens, as well as other cellular functions. These results provide novel insights to the molecular control of chickpea cellular processes, which may assist the understanding of chickpea defence mechanisms and allow enhanced development of disease resistant cultivars.
利用微阵列技术以及一组鹰嘴豆（Cicer arietinum L.）单基因和草豌豆（Lathyrus sativus L.）表达序列标签，在三种对褐斑病[Ascochyta rabiei (Pass.) L.]具有不同抗性水平的鹰嘴豆基因型中，研究了鹰嘴豆对防御信号化合物水杨酸（SA）、茉莉酸甲酯（MeJA）和氨基环丙烷羧酸（ACC）处理的反应。该实验系统将环境影响降至最低，并采用参照设计进行，其中未处理对照的样本作为处理后样本的参照。微阵列观察结果也通过定量逆转录 - 聚合酶链反应进行了验证。715个实验微阵列特征的时间进程表达模式导致425个转录本差异表达。对褐斑病具有抗性的鹰嘴豆基因型在所有处理下均表现出更广泛的与防御相关的基因诱导，表明它们可能具有更强的抵抗病原体的能力。此外，还确定了SA、MeJA和ACC信号传导参与了一些重要的对褐斑病有反应的转录本的调控，以及这些途径之间的相互作用。在本研究中，我们还发现证据表明存在褐斑病特异性信号传导机制参与诱导一些先前与褐斑病抗性相关的转录本。这项研究表征了许多鹰嘴豆转录本的调控机制，这些机制可能在抵御各种病原体以及其他细胞功能方面具有重要作用。这些结果为鹰嘴豆细胞过程的分子控制提供了新的见解，这可能有助于理解鹰嘴豆的防御机制，并促进抗病品种的改良。
