人胎盘通过抑制凋亡因子和促进毛囊增殖诱导化疗引起的脱发再生。
Human placenta induces hair regrowth in chemotherapy-induced alopecia via inhibition of apoptotic factors and proliferation of hair follicles.
机构信息
Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea.
Department of Ophthalmology, Otorhinolaryngology and Dermatology of Korean Medicine, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.
出版信息
BMC Complement Med Ther. 2020 Jul 20;20(1):230. doi: 10.1186/s12906-020-03025-z.
BACKGROUND
The human placenta (HP) is a complex organ used to alleviate tiredness and promote wound healing. Previous research showed the hair growth-promoting effect of HP. However, no reports have addressed the effects of HP on hair regrowth in chemotherapy-induced alopecia. In this study, we investigated the effects of HP on the apoptosis and proliferation of hair follicles in chemotherapy-induced alopecia.
METHODS
Male C57BL/6 mice in telogen were depilated to enter anagen. After 9 days, dystrophic catagen was induced by the intraperitoneal injection of 150 mg/kg cyclophosphamide. During 9 to 16 days, 0.1 and 1 mg/mL HP were topically applied to depilated dorsal skin.
RESULTS
Dystrophic hair follicles by cyclophosphamide were recovered by HP treatment. New hair shafts containing hair fibers appeared to be straight after HP treatment. Immunohistological staining revealed a significant increase of Ki67-positive cells in hair follicles treated with 1 mg/mL HP. Topical HP treatment increased the ratio of Bcl-2/Bax, while it attenuated the expression of pro-apoptotic Bax, p53, and cytochrome c with caspase-9 and -3. In addition, the expression of KGF and the phosphorylation of AKT were upregulated by HP treatment.
CONCLUSION
These results suggest that HP treatment induced hair growth by inhibiting apoptosis and promoting the proliferation of hair follicles. HP may be useful for treating chemotherapy-induced alopecia.
背景
胎盘（HP）是一种复杂的器官，用于缓解疲劳和促进伤口愈合。先前的研究表明 HP 具有促进毛发生长的作用。然而，目前尚无关于 HP 对化疗引起的脱发中毛发生长的影响的报道。在这项研究中，我们研究了 HP 对化疗诱导性脱发中毛囊凋亡和增殖的影响。
方法
处于休止期的雄性 C57BL/6 小鼠被剃毛以进入生长期。9 天后，通过腹腔注射 150mg/kg 的环磷酰胺诱导退行期。在 9 到 16 天期间，将 0.1 和 1mg/mL 的 HP 局部应用于剃毛的背部皮肤。
结果
HP 处理可恢复环磷酰胺引起的退行性毛囊。经 HP 处理后，出现的新毛干似乎是直的。免疫组织化学染色显示，用 1mg/mL HP 处理的毛囊中 Ki67 阳性细胞显著增加。局部 HP 处理增加了 Bcl-2/Bax 的比值，同时减弱了促凋亡 Bax、p53 和细胞色素 c 与 caspase-9 和 -3 的表达。此外，HP 处理上调了 KGF 的表达和 AKT 的磷酸化。
结论
这些结果表明，HP 处理通过抑制凋亡和促进毛囊增殖来诱导毛发生长。HP 可能对治疗化疗引起的脱发有用。
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