内皮细胞和红细胞微粒与中国急性心肌梗死的关系：一项回顾性研究。

Association of endothelial and red blood cell microparticles with acute myocardial infarction in Chinese: a retrospective study.

机构信息

Department of Cardiology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China.

Xinjiang Emergency Center, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China.

出版信息

Ann Palliat Med. 2020 Jul;9(4):1564-1570. doi: 10.21037/apm-20-397. Epub 2020 Jul 20.

DOI:10.21037/apm-20-397

PMID:32692196

Abstract

BACKGROUND

Mounting evidence suggests that endothelial cell-derived microparticles (EMPs) and red blood cell-derived microparticles (RMPs), which have procoagulant and vasoconstriction effects, are involved in the development of vascular acute cardiovascular events. The aim of this study was to analyze the circulating levels of EMPs and RMPs in patients with acute myocardial infarction (AMI), and to explore the correlations between EMPs and RMPs and the severity of coronary artery disease.

METHODS

Plasma samples from 110 patients with AMI and 57 non-coronary artery disease group (nonCAD) were collected in the present study. The flow cytometry was used to measure the EMPs (CD31) and RMPs (CD235a) qualitatively and quantitatively.

RESULTS

The levels of EMPs and RMPs in the AMI group were higher than that in the Non-CAD group, yet no significant difference was found between STEMI and non-STEMI subjects. The levels of EMPs and RMPs in multi-vessel were higher than in the single-vessel l disease. In the Thrombolysis in Myocardial Infarction (TIMI) risk assessment, the levels of EMPs in the high-risk group were higher than that in both intermediate- and low-risk group. The low-risk group had the lowest EMP levels, the difference between the groups being statistically significant (P=0.001). No significant difference in RMP levels was noted upon TIMI stratification. According to the ROC curve analysis, the areas under the curve (AUC) of EMPs and RMPs were 0.706 and 0.668, respectively.

CONCLUSIONS

The circulating levels of EMPs and RMPs in patients with AMI are elevated, and the level of EMPs is related to the degree of coronary artery disease and the prognosis risk. The EMPs are more likely to be potential biomarkers than RMPs to provide diagnostic value for AMI.

摘要

背景

越来越多的证据表明，内皮细胞衍生的微颗粒（EMP）和红细胞衍生的微颗粒（RMP）具有促凝和血管收缩作用，与血管急性心血管事件的发展有关。本研究旨在分析急性心肌梗死（AMI）患者循环中 EMP 和 RMP 的水平，并探讨 EMP 和 RMP 与冠状动脉疾病严重程度之间的相关性。

方法

本研究收集了 110 例 AMI 患者和 57 例非冠状动脉疾病组（nonCAD）的血浆样本。采用流式细胞术定性和定量检测 EMP（CD31）和 RMP（CD235a）。

结果

AMI 组 EMP 和 RMP 水平高于 nonCAD 组，但 STEMI 和非 STEMI 患者之间无显著差异。多支血管病变组的 EMP 和 RMP 水平高于单支血管病变组。在心肌梗死溶栓治疗（TIMI）风险评估中，高危组的 EMP 水平高于中危和低危组。低危组的 EMP 水平最低，组间差异具有统计学意义（P=0.001）。TIMI 分层时，RMP 水平无显著差异。根据 ROC 曲线分析，EMP 和 RMP 的曲线下面积（AUC）分别为 0.706 和 0.668。