Zhejiang University, College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agri-Food Processing, Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Hangzhou 310058, People's Republic of China.
G.B. Pant National Institute of Himalayan Environment, Kosi- Katarmal, Almora 263643, India; Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India.
Biotechnol Adv. 2020 Nov 1;43:107600. doi: 10.1016/j.biotechadv.2020.107600. Epub 2020 Jul 18.
Anthocyanins, the color compounds of plants, are known for their wide applications in food, nutraceuticals and cosmetic industry. The biosynthetic pathway of anthocyanins is well established with the identification of potential key regulatory genes, which makes it possible to modulate its production by biotechnological means. Various biotechnological systems, including use of in vitro plant cell or tissue cultures as well as microorganisms have been used for the production of anthocyanins under controlled conditions, however, a wide range of factors affects their production. In addition, metabolic engineering technologies have also used the heterologous production of anthocyanins in recombinant plants and microorganisms. However, these approaches have mostly been tested at the lab- and pilot-scales, while very few up-scaling studies have been undertaken. Various challenges and ways of investigation are proposed here to improve anthocyanin production by using the in vitro plant cell or tissue culture and metabolic engineering of plants and microbial culture systems. All these methods are capable of modulating the production of anthocyanins , which can be further utilized for pharmaceutical, cosmetics and food applications.
花色苷是植物中的颜色化合物，因其在食品、营养保健品和化妆品行业的广泛应用而闻名。花色苷的生物合成途径已经得到很好的确定，并且已经鉴定出了潜在的关键调控基因，这使得通过生物技术手段调节其产量成为可能。各种生物技术系统，包括使用体外植物细胞或组织培养以及微生物，已经被用于在控制条件下生产花色苷，然而，广泛的因素会影响其生产。此外，代谢工程技术也已经在重组植物和微生物中使用异源生产花色苷。然而，这些方法大多只在实验室和中试规模进行了测试，而很少进行放大研究。这里提出了各种挑战和研究方法，以通过体外植物细胞或组织培养和植物及微生物培养系统的代谢工程来提高花色苷的产量。所有这些方法都能够调节花色苷的产生，这些方法可以进一步用于制药、化妆品和食品应用。
