Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, PR China; Oil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Wuhan 430062, PR China.
CSIRO Agriculture & Food, Canberra 2601, Australia.
Prog Lipid Res. 2021 Jan;81:101083. doi: 10.1016/j.plipres.2020.101083. Epub 2020 Dec 26.
There is an increasing demand for astaxanthin in food, feed, cosmetics and pharmaceutical applications because of its superior anti-oxidative and coloring properties. However, naturally produced astaxanthin is expensive, mainly due to low productivity and limited sources. Reprogramming of microorganisms for astaxanthin production via metabolic engineering is a promising strategy. We primarily focus on the application of synthetic biology, enzyme engineering and metabolic engineering in enhancing the synthesis and accumulation of astaxanthin in microorganisms in this review. We also discuss the biosynthetic pathways of astaxanthin within natural producers, and summarize the achievements and challenges in reprogramming microorganisms for enhancing astaxanthin production. This review illuminates recent biotechnological advances in microbial production of astaxanthin. Future perspectives on utilization of new technologies for boosting microbial astaxanthin production are also discussed.
由于其卓越的抗氧化和着色性能，虾青素在食品、饲料、化妆品和制药应用中的需求不断增加。然而，由于生产力低和来源有限，天然虾青素价格昂贵。通过代谢工程对微生物进行虾青素生产的重新编程是一种很有前途的策略。在本综述中，我们主要关注合成生物学、酶工程和代谢工程在增强微生物中虾青素的合成和积累方面的应用。我们还讨论了天然生产者中虾青素的生物合成途径，并总结了重新编程微生物以提高虾青素产量的成就和挑战。本综述阐明了微生物生产虾青素的最新生物技术进展。还讨论了利用新技术提高微生物虾青素产量的未来展望。
