Liou Jong-Shian, Huang Chien-Hsun, Ikeyama Nao, Lee Ai-Yun, Chen I-Ching, Blom Jochen, Chen Chih-Chieh, Chen Chia-Hsuan, Lin Yu-Chun, Hsieh Sung-Yuan, Huang Lina, Ohkuma Moriya, Watanabe Koichi, Sakamoto Mitsuo
Bioresource Collection and Research Center, Food Industry Research and Development Institute, 331 Shih-Pin Rd, Hsinchu 30062, Taiwan, ROC.
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan.
Int J Syst Evol Microbiol. 2020 Aug;70(8):4767-4773. doi: 10.1099/ijsem.0.004342.
A strictly anaerobic predominant bacterium, designated as strain gm001, was isolated from a freshly voided faecal sample collected from a healthy Taiwanese adult. Cells were Gram-stain-negative rods, non-motile and non-spore-forming. Strain gm001 was identified as a member of the genus , and a comparison of 16S rRNA and gene sequences revealed sequence similarities of 98.5 and 93.3 %, respectively, demonstrating that it was most closely related to the type strain of . Phylogenomic tree analysis indicated that the gm001 cluster is an independent lineage of DSM 18205. The average nucleotide identity, digital DNA‒DNA hybridization and average amino acid identity values between strain gm001 and DSM 18205 were 80.9, 28.6 and 83.8 %, respectively, which were clearly lower than the species delineation thresholds. The species-specific genes of this novel species were also identified on the basis of pan-genomic analysis. The predominant menaquinones were MK-11 and MK-12, and the predominant fatty acids were anteiso-C, C and iso-C. Acetate and succinate were produced from glucose as metabolic end products. Taken together, the results indicate that strain gm001 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is gm001 (=BCRC 81118=JCM 33280).
从一名健康台湾成年人新鲜排出的粪便样本中分离出一种严格厌氧的优势细菌,命名为菌株gm001。细胞为革兰氏阴性杆菌,无运动性,不形成芽孢。菌株gm001被鉴定为该属的一个成员,16S rRNA和基因序列比较分别显示序列相似性为98.5%和93.3%,表明它与该属模式菌株关系最为密切。系统基因组树分析表明,gm001聚类是DSM 18205的一个独立谱系。菌株gm001与DSM 18205之间的平均核苷酸同一性、数字DNA-DNA杂交和平均氨基酸同一性值分别为80.9%、28.6%和83.8%,明显低于物种界定阈值。基于泛基因组分析也鉴定出了该新物种的物种特异性基因。主要的甲基萘醌是MK-11和MK-12,主要脂肪酸是anteiso-C、C和iso-C。葡萄糖代谢产生的代谢终产物为乙酸盐和琥珀酸盐。综合来看,结果表明菌株gm001代表该属的一个新物种,为此提出新名称sp. nov.。模式菌株为gm001(=BCRC 81118=JCM 33280)。