Corrêa Monica Grazieli, Ribeiro Fernanda Vieira, Pimentel Suzana Peres, Benatti Bruno Braga, Felix Silva Pedro Henrique, Casati Marcio Zaffalon, Cirano Fabiano Ribeiro
Dental Research Division, School of Dentistry, Paulista University, São Paulo, Brazil.
School of Dentistry, Federal University of Maranhão, São Luís, Brazil.
Acta Odontol Scand. 2021 Apr;79(3):174-181. doi: 10.1080/00016357.2020.1797159. Epub 2020 Jul 22.
Investigate the impact of resveratrol (RESV) on peri-implant repair and its effect on bone-related markers in rats with induced diabetes mellitus (DM).
Ninety rats were divided into: DM + RESV ( = 18); DM + placebo (PLAC) ( = 18); DM + insulin (INS) ( = 18); DM + RESV + INS ( = 18); Non-DM ( = 18). Diabetes was induced by streptozotocin. One screw-shaped titanium implant was inserted in each tibiae of animals. Treatments were administered during 30 days. After, one of the implants was removed for counter-torque and the peri-implant tissue was collected for mRNA quantification of BMP-2, OPN, Runx2, Lrp-5, Osx, β-catenin, Dkk1, OPG, and RANKL by Real-time PCR. The other tibia was submitted to MicroCT analysis to measure: bone volume (BV/TV), trabecular thickness (Tb.Th) and bone-implant contact (BIC).
Higher counter-torque values were observed for implant removal in DM + RESV, DM + RESV + INS and Non-DM groups when compared to DM + PLAC ( < .05). Augmented Tb.Th was observed in DM + RESV and Non-DM when compared to DM + PLAC group ( < .05), whereas higher BIC was detected in DM + RESV, DM + RESV + INS and Non-DM animals when compared to DM + PLAC ( < .05). Levels of RANKL were downregulated by the RESV and/or INS therapy, whereas only the association of RESV and INS upregulated the levels of Runx2 ( < .05).
The therapy with RESV may favour peri-implant bone repair improving bone formation around implants.
研究白藜芦醇（RESV）对诱导型糖尿病（DM）大鼠种植体周围修复的影响及其对骨相关标志物的作用。
将90只大鼠分为：糖尿病+白藜芦醇组（n = 18）；糖尿病+安慰剂组（PLAC）（n = 18）；糖尿病+胰岛素组（INS）（n = 18）；糖尿病+白藜芦醇+胰岛素组（n = 18）；非糖尿病组（n = 18）。通过链脲佐菌素诱导糖尿病。在动物的每只胫骨中植入一枚螺旋形钛种植体。治疗持续30天。之后，取出一枚种植体测量抗扭矩，并收集种植体周围组织，通过实时聚合酶链反应对骨形态发生蛋白-2（BMP-2）、骨桥蛋白（OPN）、Runx2、低密度脂蛋白受体相关蛋白5（Lrp-5）、成骨细胞特异性转录因子（Osx）、β-连环蛋白、 Dickkopf-1（Dkk1）、骨保护素（OPG）和核因子κB受体活化因子配体（RANKL）进行mRNA定量分析。对另一只胫骨进行显微CT分析，测量骨体积（BV/TV）、骨小梁厚度（Tb.Th）和骨-种植体接触率（BIC）。
与糖尿病+安慰剂组相比，糖尿病+白藜芦醇组、糖尿病+白藜芦醇+胰岛素组和非糖尿病组在种植体取出时观察到更高的抗扭矩值（P <.05）。与糖尿病+安慰剂组相比，糖尿病+白藜芦醇组和非糖尿病组观察到骨小梁厚度增加（P <.05），而与糖尿病+安慰剂组相比，糖尿病+白藜芦醇组、糖尿病+白藜芦醇+胰岛素组和非糖尿病组动物检测到更高的骨-种植体接触率（P <.05）。白藜芦醇和/或胰岛素治疗下调了RANKL水平，而只有白藜芦醇和胰岛素联合使用上调了Runx2水平（P <.05）。
白藜芦醇治疗可能有利于种植体周围骨修复，改善种植体周围的骨形成。
