Dental Research Division, School of Dentistry, Paulista University, São Paulo, São Paulo, Brazil.
Department of Surgery and Bucco-Maxillofacial Traumatology and Periodontology, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Clin Oral Implants Res. 2019 Jan;30(1):1-10. doi: 10.1111/clr.13384. Epub 2018 Dec 26.
Innovative approaches capable to improve peri-implant bone repair are relevant in the presence of smoking, a risk factor for healing around implants. This study investigated the effect of resveratrol (RESV) on peri-implant repair, and its influence on bone-related markers in rats submitted to cigarette smoking inhalation (CSI).
One titanium implant was inserted in each tibiae of rats assigned to CSI+RESV (n:18); CSI+placebo (n:18); and non-CSI (n:18). One implant was removed for counter torque, and the peri-implant tissue was collected for mRNA quantification of BMP-2, OPN, Runx2, Lrp-5, Osx, β-catenin, Dkk1, OPG, and RANKL. The other tibia was submitted to microCT to measure: bone volume, bone porosity, trabecular spacing, trabecular thickness, and bone-implant contact (BIC).
No differences were detected between counter torque in CSI+RESV and non-CSI group (p > 0.05), whereas CSI+placebo group presented lower values when compared to the others (p < 0.05). RESV improved the BIC in CSI rats without differences when compared to non-CSI group (p > 0.05), whereas CSI+placebo showed reduced BIC when compared to the other groups (p < 0.05). RESV reduced RANKL/OPG and Lrp-5 levels and increased β-catenin in CSI rats when compared to CSI+placebo (p < 0.05).
Although further investigations should be considered using oral models of dental implants, within the limits of the present study, it was concluded that RESV reverses the negative effects of smoking in the peri-implant repair, benefiting the modulation of bone-related markers.
在吸烟（种植体周围愈合的危险因素）存在的情况下，能够改善种植体周围骨修复的创新方法是相关的。本研究调查了白藜芦醇（RESV）对种植体周围修复的影响，及其对吸烟吸入（CSI）大鼠骨相关标志物的影响。
将一个钛种植体插入每组大鼠的胫骨中，分别为 CSI+RESV（n：18）；CSI+安慰剂（n：18）；非 CSI（n：18）。一个种植体用于反扭矩去除，收集种植体周围组织，用于 BMP-2、OPN、Runx2、Lrp-5、Osx、β-catenin、Dkk1、OPG 和 RANKL 的 mRNA 定量。另一个胫骨进行 microCT 测量：骨体积、骨孔隙率、小梁间隔、小梁厚度和骨-种植体接触（BIC）。
CSI+RESV 与非 CSI 组的反扭矩无差异（p>0.05），而 CSI+安慰剂组与其他组相比，值较低（p<0.05）。RESV 改善了 CSI 大鼠的 BIC，与非 CSI 组无差异（p>0.05），而 CSI+安慰剂组与其他组相比，BIC 降低（p<0.05）。RESV 降低了 CSI 大鼠的 RANKL/OPG 和 Lrp-5 水平，增加了β-catenin，与 CSI+安慰剂组相比（p<0.05）。
尽管应该考虑使用口腔种植体模型进行进一步研究，但在本研究的范围内，可以得出结论，RESV 逆转了吸烟对种植体周围修复的负面影响，有利于骨相关标志物的调节。
