Hwang Hyejung, Mizuno Sahiro, Kasai Nobukazu, Kojima Chihiro, Sumi Daichi, Hayashi Nanako, Goto Kazushige
Phys Act Nutr. 2020 Jun 30;24(2):30-37. doi: 10.20463/pan.2020.0012.
The present study investigated the effect of endurance exercise with blood flow restriction (BFR) performed at either 25% maximal oxygen uptake (V˙O2 max) or 40% V˙O2 max) on muscle oxygenation, energy metabolism, and endocrine responses.
Ten males were recruited in the present study. The subjects performed three trials: (1) endurance exercise at 40% V˙O2 max without BFR (NBFR40), (2) endurance exercise at 25% V˙O2 max with BFR (BFR25), and (3) endurance exercise at 40% V˙O2 max with BFR (BFR40). The exercises were performed for 15 min during which the pedaling frequency was set at 70 rpm. In BFR25 and BFR40, 2 min of pressure phase (equivalent to 160 mmHg) followed by 1 min of release phase were repeated five times (5 × 3 min) throughout 15 minutes of exercise. During exercise, muscle oxygenation and concentration of respiratory gases were measured. The blood samples were collected before exercise, immediately after 15 min of exercise, and at 15, 30, and 60 minutes after completion of exercise.
Deoxygenated hemoglobin (deoxy-Hb) level during exercise was significantly higher with BFR25 and BFR40 than that with NBFR40. BFR40 showed significantly higher total-hemoglobin (total-Hb) than NBFR40 during 2 min of pressure phase. Moreover, exercise-induced lactate elevation and pH reduction were significantly augmented in BFR40, with concomitant increase in serum cortisol concentration after exercise. Carbohydrate (CHO) oxidation was significantly higher with BFR40 than that with NBFR40 and BFR25, whereas fat oxidation was lower with BFR40.
Deoxy-Hb and total Hb levels were significantly increased during 15 min of pedaling exercise in BFR25 and BFR40, indicating augmented local hypoxia and blood volume (blood perfusion) in the muscle. Moreover, low-and moderate-intensity exercise with BFR facilitated CHO oxidation.
本研究调查了在25%最大摄氧量（V˙O2 max）或40% V˙O2 max下进行的限制血流（BFR）耐力运动对肌肉氧合、能量代谢和内分泌反应的影响。
本研究招募了10名男性。受试者进行了三项试验：（1）在40% V˙O2 max下进行无BFR的耐力运动（NBFR40），（2）在25% V˙O2 max下进行有BFR的耐力运动（BFR25），以及（3）在40% V˙O2 max下进行有BFR的耐力运动（BFR40）。运动持续15分钟，在此期间踏板频率设定为70转/分钟。在BFR25和BFR40组中，在整个15分钟的运动过程中，2分钟的加压阶段（相当于160 mmHg）后接着1分钟的减压阶段重复5次（5×3分钟）。运动期间，测量肌肉氧合和呼吸气体浓度。在运动前、运动15分钟后立即以及运动结束后15、30和60分钟采集血样。
与NBFR40相比，BFR25和BFR40运动期间的脱氧血红蛋白（deoxy-Hb）水平显著更高。在加压阶段的2分钟内，BFR40的总血红蛋白（total-Hb）显著高于NBFR40。此外，BFR40运动诱导的乳酸升高和pH降低显著增强，运动后血清皮质醇浓度随之增加。与NBFR40和BFR25相比，BFR40的碳水化合物（CHO）氧化显著更高，而BFR40的脂肪氧化更低。
在BFR25和BFR40中，15分钟的踏板运动期间脱氧-Hb和总Hb水平显著增加，表明肌肉局部缺氧和血容量（血液灌注）增加。此外，低强度和中等强度的BFR运动促进了CHO氧化。
