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从蜂蜜和蜜蜂微生物群中分离出的两种潜在益生菌枯草芽孢杆菌的全基因组序列及比较分析

Complete genome sequence and comparative analysis of two potential probiotics Bacillus subtilis isolated from honey and honeybee microbiomes.

作者信息

Hamdy Abdelhamid A, Esawy Mona A, Elattal Nouran A, Amin Magdy A, Ali Amal E, Awad Ghada E A, Connerton Ian, Mansour Nahla M

机构信息

Chemistry of Natural and Microbial Products Department, Pharmaceutical Industries Research Div., National Research Centre, 33 El Bohouth St., Dokki, P.O. Box: 12622, Cairo, Egypt.

Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

出版信息

J Genet Eng Biotechnol. 2020 Jul 22;18(1):34. doi: 10.1186/s43141-020-00050-w.

DOI:10.1186/s43141-020-00050-w
PMID:32700263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7376800/
Abstract

BACKGROUND

We have previously isolated Bacillus subtilis HMNig-2 and MENO2 strains, from honey and the honeybee gut microbiome respectively, and demonstrated these strains to produce levansucrase with potential probiotics characteristics. Here we report their complete genome sequence and comparative analysis with other and other B. subtilis strains.

RESULTS

The complete genome sequences of Bacillus subtilis HMNig-2 and MENO2 were de novo assembled from MiSeq paired-end sequence reads and annotated using the RAST tool. Whole-genome alignments were performed to identify functional differences associated with their potential use as probiotics.

CONCLUSIONS

The comparative analysis and the availability of the genome sequence of these two strains will provide comprehensive analysis about the diversity of these valuable Bacillus strains and the possible impact of the environment on bacterial evolution.

SIGNIFICANCE AND IMPACT OF STUDY

We introduce complete genome sequence of two new B. subtilis strains HMNig-2 and MENO2 with probiotic potential and as cell factories for the production of levan and other valuable components for pharmaceutical and industrial applications.

摘要

背景

我们之前分别从蜂蜜和蜜蜂肠道微生物群中分离出枯草芽孢杆菌HMNig - 2和MENO2菌株，并证明这些菌株可产生具有潜在益生菌特性的蔗糖酶。在此，我们报告它们的完整基因组序列以及与其他枯草芽孢杆菌菌株的比较分析。

结果

枯草芽孢杆菌HMNig - 2和MENO2的完整基因组序列通过MiSeq双端序列读数进行了从头组装，并使用RAST工具进行注释。进行全基因组比对以确定与其作为益生菌的潜在用途相关的功能差异。

结论

这两种菌株的比较分析和基因组序列的可得性将为这些有价值的芽孢杆菌菌株的多样性以及环境对细菌进化的可能影响提供全面分析。

研究的意义和影响

我们介绍了两种具有益生菌潜力的新型枯草芽孢杆菌菌株HMNig - 2和MENO2的完整基因组序列，它们可作为生产果聚糖及其他用于制药和工业应用的有价值成分的细胞工厂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9da7/7376800/989f9592e990/43141_2020_50_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9da7/7376800/9b0d810e9718/43141_2020_50_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9da7/7376800/f9847edef3fc/43141_2020_50_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9da7/7376800/989f9592e990/43141_2020_50_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9da7/7376800/9b0d810e9718/43141_2020_50_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9da7/7376800/f9847edef3fc/43141_2020_50_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9da7/7376800/989f9592e990/43141_2020_50_Fig3_HTML.jpg

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