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Bbv57 的全基因组序列分析，一种有前途的植物病原菌生防剂。

Complete Genome Sequence Analysis of Bbv57, a Promising Biocontrol Agent against Phytopathogens.

机构信息

Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India.

Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India.

出版信息

Int J Mol Sci. 2022 Aug 27;23(17):9732. doi: 10.3390/ijms23179732.

DOI:10.3390/ijms23179732
PMID:36077128
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9456384/
Abstract

Plant growth-promoting rhizobacteria (PGPR) are a group of root-associated beneficial bacteria emerging as one of the powerful agents in sustainable plant disease management. Among the PGPR, Bacillus sp. has become a popular biocontrol agent for controlling pests and the diseases of several crops of agricultural and horticultural importance. Understanding the molecular basis of the plant growth-promoting and biocontrol abilities of Bacillus spp. will allow us to develop multifunctional microbial consortia for sustainable agriculture. In our study, we attempted to unravel the genome complexity of the potential biocontrol agent Bacillus subtilis Bbv57 (isolated from the betelvine’s rhizosphere), available at TNAU, Coimbatore. A WGS analysis generated 26 million reads, and a de novo assembly resulted in the generation of 4,302,465 bp genome of Bacillus subtilis Bbv57 containing 4363 coding sequences (CDS), of which 4281 were functionally annotated. An analysis of 16S rRNA revealed its 100% identity to Bacillus subtilis IAM 12118. A detailed data analysis identified the presence of >100 CAZymes and nine gene clusters involved in the production of secondary metabolites that exhibited antimicrobial properties. Further, Bbv57 was found to harbor 282 unique genes in comparison with 19 other Bacillus strains, requiring further exploration.

摘要

植物促生根际细菌（PGPR）是一组与根相关的有益细菌，作为可持续植物病害管理的有力手段之一而崭露头角。在 PGPR 中，芽孢杆菌属已成为控制害虫和几种重要农业和园艺作物病害的热门生物防治剂。了解芽孢杆菌属的促生和生物防治能力的分子基础，将使我们能够开发多功能微生物群落，以实现可持续农业。在我们的研究中，我们试图揭示潜在生物防治剂枯草芽孢杆菌 Bbv57（从菝葜的根际中分离得到）的基因组复杂性，该菌株可在泰米尔纳德邦农业大学（TNAU），哥印拜陀获得。全基因组测序分析产生了 2600 万条reads，从头组装生成了 4302465bp 的枯草芽孢杆菌 Bbv57 基因组，其中包含 4363 个编码序列（CDS），其中 4281 个具有功能注释。16S rRNA 分析表明其与枯草芽孢杆菌 IAM 12118 的相似度为 100%。详细数据分析确定了存在>100 个 CAZymes 和九个参与产生具有抗菌特性的次生代谢物的基因簇。此外，与其他 19 株芽孢杆菌相比，Bbv57 中存在 282 个独特基因，这需要进一步探索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7f7/9456384/2a6a29db484f/ijms-23-09732-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e7f7/9456384/281900b651d8/ijms-23-09732-g001.jpg
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