Department of Biological Sciences, University of Calgary, 2500 University Drive N.W., Calgary, Alberta, T2N N4, Canada.
Chembiochem. 2021 Jan 15;22(2):264-287. doi: 10.1002/cbic.202000354. Epub 2020 Sep 7.
Pathogenesis-related (PR) proteins constitute a broad class of plant proteins with analogues found throughout nature from bacteria to higher eukaryotes. PR proteins were first noted in plants as part of the hypersensitive response, but have since been assigned an array of biological roles. The PR10/Bet v1-like proteins are a subset of PR proteins characterized by an ability to bind a wide range of lipophilic ligands, uniquely positioning them as contributors to specialized biosynthetic pathways. PR10/Bet v1-like proteins participate in the production of plant alkaloids and phenolics including flavonoids, both as general binding proteins and in special cases as catalysts. Owing initially to the perceived allergenic properties of PR10/Bet v1-like proteins, many were studied at the structural level to elucidate the basis for ligand binding. These studies provided a foundation for more recent efforts to understand higher-level structural order and how PR10/Bet v1-like proteins catalyse key reactions in plant pathways. Synthetic biology aimed at reconstituting plant-specialized metabolism in microorganisms uses knowledge of these proteins to fine-tune performance in new systems.
植物凝集素（PR）蛋白是一大类植物蛋白，从细菌到高等真核生物，自然界中都存在类似物。PR 蛋白最初被认为是植物过敏反应的一部分，但后来被赋予了一系列生物学功能。PR10/Bet v1 类蛋白是 PR 蛋白的一个亚类，其特点是能够结合广泛的亲脂性配体，这使它们成为专门生物合成途径的贡献者。PR10/Bet v1 类蛋白参与植物生物碱和酚类的产生，包括类黄酮，它们既是通用结合蛋白，在特殊情况下又是催化剂。最初由于 PR10/Bet v1 类蛋白被认为具有过敏原特性，因此许多人从结构水平上对其进行了研究，以阐明配体结合的基础。这些研究为理解更高层次的结构顺序以及 PR10/Bet v1 类蛋白如何催化植物途径中的关键反应奠定了基础。旨在在微生物中重新构建植物特有的代谢途径的合成生物学利用这些蛋白质的知识来微调新系统中的性能。
