L. 叶提取物对γ射线辐射诱导的氧化应激的保护作用。
Protective Role of L. Leaves Extract against Gamma-irradiation-induced Oxidative Stress.
出版信息
Pak J Biol Sci. 2020 Jan;23(7):922-930. doi: 10.3923/pjbs.2020.922.930.
BACKGROUND AND OBJECTIVE
Radiation exposure can cause several harmful effects in biological systems due to free radical production. Several antioxidants have been tested as potential hepatoprotective agents against ionizing radiation as they lower oxidative stress in normal cells induced by Reactive Oxygen Species (ROS). The present study was conducted to evaluate the possible ameliorative effects of Juniperus phoenicea L.
MATERIALS AND METHODS
Aqueous leaves extract on different biochemical and histopathological parameters against whole body gamma-irradiation-induced oxidative stress, organ dysfunction and metabolic disturbances in experimental Swiss Albino rats. After a single dose of gamma-radiation (6 Gy), there was a significant reduction in albumin, total protein and globulin levels and a significant increase in the liver enzymes (ALT, AST, ALP and GGT) and lipid profile parameters (cholesterol, triglyceride, HDL-cholesterol and LDL-cholesterol) in gamma-irradiated rats unlike in normal controls.
RESULTS
The gamma-irradiated rats pre-treated with J. phoenicea leaf extracts, however, showed a significant increase in albumin, total protein and globulin levels and a significant reduction in liver enzymes and lipid profile parameters as opposed to the untreated ones. The gamma-irradiated rats showed toxic changes in the liver, whereas, the rats pre-treated with J. phoenicea leaves extract demonstrated a protective effect. Additionally, gamma- irradiation caused myocardial degenerative changes, interstitial edema between muscle fibers, necrosis and inflammatory cells infiltration and fibrotic and cellular damages to the heart, but J. phoenicea leaves extract were found to ameliorate the gamma-irradiation-induced changes in the heart.
CONCLUSION
The results suggested that treatment with J. phoenicea leaves extract is possibly safe and can ameliorate gamma-irradiation-induced oxidative damage and tissue injury in rats. The leaves of J. phoenicea could serve as a potential source of therapeutic antioxidants.
背景与目的
由于自由基的产生，辐射会对生物系统造成多种有害影响。许多抗氧化剂已被测试为潜在的放射防护剂，因为它们可以降低活性氧（ROS）诱导的正常细胞中的氧化应激。本研究旨在评估 Juniperus phoenicea L. 叶水提物对全身体γ射线照射诱导的氧化应激、器官功能障碍和代谢紊乱的可能改善作用，实验采用瑞士白化大鼠。
材料与方法
在单次全身γ射线照射（6 Gy）后，与正常对照组相比，γ射线照射大鼠的白蛋白、总蛋白和球蛋白水平显著降低，而肝酶（ALT、AST、ALP 和 GGT）和脂质谱参数（胆固醇、甘油三酯、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇）显著升高。与未治疗的大鼠相比，用 J. phoenicea 叶提取物预处理的γ射线照射大鼠白蛋白、总蛋白和球蛋白水平显著升高，肝酶和脂质谱参数显著降低。γ射线照射大鼠的肝脏出现毒性变化，而用 J. phoenicea 叶提取物预处理的大鼠则表现出保护作用。此外，γ 射线照射导致心肌退行性变化、肌纤维间间质水肿、坏死和炎性细胞浸润以及心脏纤维化和细胞损伤，但 J. phoenicea 叶提取物可改善γ射线照射引起的心脏变化。
结论
结果表明，用 J. phoenicea 叶提取物治疗可能是安全的，可以改善大鼠的γ射线照射诱导的氧化损伤和组织损伤。J. phoenicea 的叶子可能是治疗抗氧化剂的潜在来源。
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