Integrated Bioprocessing Laboratory, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology , Kattankulathur, India.
Laboratoire de génie de l'environnement, Faculté de génie, Université de Sherbrooke, 2500 boul. de l'Université , Sherbrooke, Québec, Canada.
J Air Waste Manag Assoc. 2020 Dec;70(12):1252-1259. doi: 10.1080/10962247.2020.1760958.
Lignin obtained from renewable biomass is a potential feedstock for the synthesis of various value-added chemicals through efficient biocatalytic routes. The ligninolytic enzymes-assisted depolymerization of lignin to vanillin constitutes the most commercially attractive and promising approach in green chemistry as vanillin constitutes the second most prevalent flavoring agent. Thus, in the present work, immobilized laccase and versatile peroxidase, and further, a co-immobilized laccase and versatile peroxidase system on magnetic silica microspheres (MSMS) were developed to generate a robust biocatalytic system that mediates the depolymerization of lignin obtained from biomass. The depolymerization of lignin by free and immobilized laccase showed a vanillin yield of 24.8% and 23%, respectively, at pH 4.0 in 6 h at 30°C against a vanillin yield of 20% and 21.7% with the free and immobilized versatile peroxidase, respectively, at pH 5.0°C and 50°C. Comparatively, the system with the co-immobilized laccase and versatile peroxidase exhibited a 1-fold and 1.2-fold higher vanillin yield than the free and immobilized laccase system, respectively. On comparing with the versatile peroxidase system, the co-immobilized biocatalytic system displayed 1.3-fold and 1.2-fold increased vanillin yield than the free and immobilized versatile peroxidase system, respectively, at a pH of 6.0 in 6 h at 30°C with an enzyme concentration of 1 U/ml. The reusability studies of the co-immobilized biocatalytic system exhibited that both the enzymes retained up to 40% of its activity till sixth cycle. : The waste biomass of is widely available around the coastal regions of India which does not have any agricultural or industrial applications. The present work exploits the lignocellulosic content of the biomass to extract the lignin, which provides a renewable alternative for the production of the commercially high-valued compound, vanillin. This work also integrates a co-immobilized biocatalytic process comprising of laccase and versatile peroxidase leading to an environmentally benign enzymatic process for the depolymerization of lignin to vanillin.
从可再生生物质中获得的木质素是通过高效生物催化途径合成各种增值化学品的潜在原料。木质素解聚酶辅助木质素解聚为香草醛构成了绿色化学中最具商业吸引力和前景的方法，因为香草醛是第二大常见的香料。因此，在本工作中，固定化漆酶和多功能过氧化物酶，以及进一步在磁性硅胶微球（MSMS）上共固定化漆酶和多功能过氧化物酶系统，被开发用于产生介导生物质中木质素解聚的强大生物催化系统。游离和固定化漆酶对木质素的解聚在 30°C 下 pH 4.0 时 6 小时分别产生 24.8%和 23%的香草醛，而游离和固定化多功能过氧化物酶分别在 pH 5.0°C 和 50°C 时产生 20%和 21.7%的香草醛。相比之下，与游离和固定化漆酶系统相比，共固定化漆酶和多功能过氧化物酶系统的香草醛产率分别提高了 1 倍和 1.2 倍。与多功能过氧化物酶系统相比，共固定化生物催化系统在 30°C 下 pH 6.0 时 6 小时内酶浓度为 1U/ml 时，分别比游离和固定化多功能过氧化物酶系统提高了 1.3 倍和 1.2 倍的香草醛产率。共固定化生物催化系统的重复使用研究表明，两种酶的活性保留至第 6 个循环，可达 40%。：印度沿海地区广泛存在的 废物生物质没有任何农业或工业用途。本工作利用 生物质的木质纤维素含量提取木质素，为商业价值高的化合物香草醛的生产提供了可再生的替代方案。这项工作还集成了一个共固定化生物催化过程，包括漆酶和多功能过氧化物酶，从而为木质素解聚为香草醛的环境友好酶促过程提供了一种选择。
