Idrees Muhammad, Ali Shakir, Rehman Abdul, Zajif Hussain Syed, Abbas Bukhari Dilara
Department of Zoology, Government College University, Lahore, Pakistan.
Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan.
Saudi J Biol Sci. 2023 Aug;30(8):103740. doi: 10.1016/j.sjbs.2023.103740. Epub 2023 Jul 13.
Due to rising populations and human activities, heavy metals (HM) toxicity has become a serious problem for all life forms. The present study deals with isolating and identifying lead-resistant bacteria from contaminated wastewater of tanneries effluents. Two isolated strains were identified as (ID1), and sp (ID3), and both strains resisted a 25 mM concentration of Lead nitrate (Pb (NO)). After four days of treatment, (ID1) showed 80% lead uptake, and sp (ID3) showed 88%. Lead uptake was confirmed by Energy dispersive X-Ray (EDX) analysis. Fourier transform infrared spectroscopy (FTIR) showed that structural alterations had occurred in functional groups of the treated samples compared to the controls. Our research indicates that these strains may be useful in bioremediating heavy metals from polluted environments. Further investigation into the processes involved in the uptake and homeostasis of heavy metals by these strains is required, as is the identification of the genes and enzymes responsible for Pb-bioremediation.
由于人口增长和人类活动，重金属（HM）毒性已成为所有生命形式面临的严重问题。本研究旨在从制革厂废水污染中分离和鉴定耐铅细菌。两种分离菌株被鉴定为（ID1）和sp（ID3），两种菌株都能耐受25 mM浓度的硝酸铅（Pb(NO)）。处理四天后，（ID1）显示出80%的铅吸收量，sp（ID3）显示出88%。通过能量色散X射线（EDX）分析确认了铅的吸收。傅里叶变换红外光谱（FTIR）表明，与对照相比，处理后样品的官能团发生了结构变化。我们的研究表明，这些菌株可能有助于从污染环境中生物修复重金属。需要进一步研究这些菌株吸收和维持重金属稳态的过程，以及鉴定负责铅生物修复的基因和酶。
