Department of Oral Biochemistry, Republic of Korea; Dental and Life Science Institute, Pusan National University School of Dentistry, Yangsan, 50612, Republic of Korea.
Department of Pediatric Dentistry, Republic of Korea; Dental Research Institute, Pusan National University Dental Hospital, Yangsan, 50612, Republic of Korea.
Biochem Biophys Res Commun. 2020 Aug 20;529(2):169-174. doi: 10.1016/j.bbrc.2020.06.013. Epub 2020 Jun 22.
Supernumerary tooth (ST) may arise from uncertain developmental abnormalities or underlying genetic causes, and the extraction at the early age is recommended. Dental pulp stem cells (DPSCs) are the valuable resource for the regeneration of tooth and related craniofacial structures. DPSCs isolated from ST (sDPSCs) have not been fully characterized despite the potential in the applications. The objectives of this study are the efficient isolation of sDPSCs and the analysis of the properties as stem cells. sDPSCs were established by hammer-cracking and separation of the intact pulp from ST. sDPSCs in the culture were examined by light microscope and flow cytometer for the morphology and the surface marker expression. sDPSCs exhibited the cellular morphology of typical mesenchymal stem cells and expressed CD44, CD73, CD90, CD105 and CD166, but not CD14, CD34 or CD45. sDPSCs showed the differentiation potential toward osteogenic, chondrogenic and adipogenic lineages. During osteogenic differentiation, the stimulation by Oncostatin M enhanced the differentiation and significantly increased the expression of genes involved in the hard tissue repair, such as BMP2, BMP4, BMP6 and RUNX2. sDPSCs can be effectively derived from ST and displays the characteristics of mesenchymal stem cells in the maintenance and the differentiation. sDPSCs satisfies the quality as DPSCs thus provide the valuable resource to the regenerative therapy.
额外牙（ST）可能是由于发育异常或潜在的遗传原因引起的，建议在早期进行拔牙。牙髓干细胞（DPSCs）是牙齿和相关颅面结构再生的宝贵资源。尽管在应用中具有潜力，但从额外牙（sDPSCs）中分离出来的 DPSCs 尚未得到充分的描述。本研究的目的是高效分离 sDPSCs 并分析其作为干细胞的特性。通过敲击和分离完整的牙髓从 ST 中建立 sDPSCs。通过显微镜和流式细胞仪检查培养中的 sDPSCs 的形态和表面标志物表达。sDPSCs 表现出典型的间充质干细胞的细胞形态，并表达 CD44、CD73、CD90、CD105 和 CD166，但不表达 CD14、CD34 或 CD45。sDPSCs 具有向成骨细胞、软骨细胞和脂肪细胞谱系分化的潜力。在成骨细胞分化过程中，Oncostatin M 的刺激增强了分化，并显著增加了参与硬组织修复的基因的表达，如 BMP2、BMP4、BMP6 和 RUNX2。sDPSCs 可以从 ST 中有效获得，并在维持和分化中表现出间充质干细胞的特征。sDPSCs 满足 DPSCs 的质量要求，因此为再生治疗提供了有价值的资源。
