Department of Otolaryngology-Head and Neck Surgery, Kobe University, Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo, 650-0017, Japan.
Department of Otolaryngology, Faculty of Medicine, The University of Tokyo, Tokyo, 113-8655, Japan.
Sci Rep. 2020 Jul 23;10(1):12271. doi: 10.1038/s41598-020-68352-0.
The symptoms of Meniere's disease (MD) are generally considered to be related to endolymphatic hydrops (EH). There are many recent reports supporting the possibility that vasopressin (VP) is closely linked to the formation of EH in Meniere's disease. Based on this, we developed a clinically relevant animal model of Meniere's disease in which a VP type 2 receptor agonist was administered after electrocauterization of the endolymphatic sac. We report live imaging of the internal structure, and functional changes of the inner ear after electrocauterization of the endolymphatic sac and administration of a VP type 2 receptor agonist. In this model, the development of EH was visualized in vivo using optical coherence tomography, there was no rupture of Reissner's membrane, and low-tone hearing loss and vertiginous attacks were observed. This study suggested that acute attacks are caused by the abrupt development of EH. This is the first report of live imaging of the development of EH induced by the administration of a VP type 2 receptor agonist.
梅尼埃病（MD）的症状通常被认为与内淋巴积水（EH）有关。有许多最近的报告支持加压素（VP）与梅尼埃病EH 的形成密切相关的可能性。基于此，我们在电灼内淋巴囊后给予 VP 型 2 受体激动剂，开发了一种与临床相关的梅尼埃病动物模型。我们报告了电灼内淋巴囊和给予 VP 型 2 受体激动剂后内耳的内部结构和功能变化的活体成像。在该模型中，使用光相干断层扫描对 EH 的发展进行了体内可视化，没有 Reissner 膜破裂，并且观察到了低频听力损失和眩晕发作。本研究表明，急性发作是由 EH 的突然发展引起的。这是首次报道 VP 型 2 受体激动剂给药诱导 EH 发展的活体成像。
