Stuart A E, Williams A R, Habeshaw J A
J Pathol. 1977 Jun;122(2):81-90. doi: 10.1002/path.1711220205.
Clustering of lymphocytes around Reed-Sternberg cells was noticed in single cell suspensions made from viable Hodgkin's lymphoid tissue. Cytocentrifugation of the suspension showed that clustering also occurred around a smaller cell type, thought to be the precursor of the classical Reed-Sternberg cell. Time-lapse cine films taken of the clustering showed unceasing activity on the part of the lymphocytes migrating over the surface of the central cell. Reed-Sternberg cells were reacted with anti-monocyte serum using indirect fluorescence techniques. In its mature form at least, the Reed-Sternberg cell showed no activity with the antiserum. No immunoglobulin was detected in the Reed-Sternberg cell using fluorescence techniques, but a few Reed-Sternberg cells showed diffuse cytoplasmic staining using the peroxidase-labelled antibody technique. Membrane receptor tests showed the lymphocytes surrounding the Reed-Sternberg cell to be T-cells. After proteolytic enzyme treatment to free lymphocytes from the surface, the Reed-Sternberg cell bound IgG-coated red blood cells indicating a probable Fc receptor. Cytochemistry demonstrated weak non-specific esterase activity in a small minority of Reed-Sternberg cells, and absence of acid phosphatase, alkaline phosphatase and peroxidase. A subpopulation of lymphocytes with distinctive segmentation of the nucleus was noted. These were often to be seen participating in lymphocyte rosettes around the Reed-Sternberg cell.
在由存活的霍奇金淋巴瘤组织制成的单细胞悬液中，观察到淋巴细胞围绕里德-斯腾伯格细胞聚集。对该悬液进行细胞离心涂片显示，在一种较小的细胞类型周围也会发生聚集，这种较小的细胞被认为是经典里德-斯腾伯格细胞的前体。对聚集过程拍摄的延时电影显示，淋巴细胞在中央细胞表面迁移时持续活跃。使用间接荧光技术让里德-斯腾伯格细胞与抗单核细胞血清反应。至少在其成熟形式下，里德-斯腾伯格细胞与抗血清无反应。使用荧光技术在里德-斯腾伯格细胞中未检测到免疫球蛋白，但使用过氧化物酶标记抗体技术时，少数里德-斯腾伯格细胞显示出弥漫性胞质染色。膜受体检测显示，围绕里德-斯腾伯格细胞的淋巴细胞为T细胞。经蛋白酶处理使淋巴细胞从表面游离后，里德-斯腾伯格细胞结合了IgG包被的红细胞，表明可能存在Fc受体。细胞化学显示，少数里德-斯腾伯格细胞有微弱的非特异性酯酶活性，且无酸性磷酸酶、碱性磷酸酶和过氧化物酶。注意到有一群细胞核有独特分叶的淋巴细胞。经常可以看到这些细胞参与围绕里德-斯腾伯格细胞的淋巴细胞玫瑰花结形成。
