Johnson Alexa C B, Rossow Heidi A
Department of Population Health and Reproduction, SVM VMTRC University of California-Davis, Tulare, CA.
Transl Anim Sci. 2018 Sep 10;3(1):340-349. doi: 10.1093/tas/txy103. eCollection 2019 Jan.
Gastrointestinal disease is the number one killer of horses. Little is known about the maintenance of microbes in the equine hindgut and how to distinguish a healthy gut in a live horse. Utilization of internal and external digestibility markers and starch fermentation has been extensively studied in ruminants and is the basis for research conducted on horses. The aims of this study were to investigate the effects of two equine feed digestive aid supplements on hindgut health () as reflected in fecal pH and digestibility and to compare and validate DM digestibility measurements through the use of internal and external markers such as chromium oxide (), lignin (), indigestible ADF (), indigestible NDF (), and indigestible lignin (). Nine mature Quarter horses (six geldings, three mares) were used in a crossover design, three feeding periods of 17 d (51 d total), using three treatments: control, no feed additive (), Smartpak (; Plymouth, MA), or Platinum Performance (; Buellton, CA). Both SP and PP contained a strain of , whereas SP further supplied mannanoligosaccharides () and fructooligosaccharides () and PP supplied Within the 17-d period, horses were offered orchard grass hay and sweet cob grain and the assigned treatment daily and four CR cookies to deliver 8 g/d of CR for the last 7 d of each period. Total feces were collected from 15 to 17 d. Feed and fecal samples were dried, ground, and sent to ANALAB (Fulton, IL) for nutrient analysis. Duplicate samples of feed and feces were placed in ruminally cannulated cows for in situ determination of iADF, iNDF, and iLig to estimate digestibility. Estimated CR fecal output, CR DMI, and DM digestibilities were evaluated using the root mean square prediction error percentage of the observed mean (), concordance correlation coefficient (), and Nash-Sutcliffe efficiency methods. Marker predictive ability tests showed iADF to have the least amount of bias with the smallest RMSPE (4%), largest CCC (0.43), and the largest amount of random bias (error of dispersion = 0.45). Supplementation of PP decreased CR DM digestibility ( < 0.02). Smartpak increased fecal pH ( < 0.09), but PP had no effect on fecal pH. Therefore, SP had a beneficial effect on HGH that is believed to be due to MOS and FOS.
胃肠道疾病是马匹的头号杀手。关于马后肠微生物的维持以及如何在活马中辨别健康的肠道,人们知之甚少。反刍动物对内部和外部消化率标记物以及淀粉发酵的利用已得到广泛研究,这也是对马进行研究的基础。本研究的目的是调查两种马用饲料消化助剂补充剂对后肠健康的影响(通过粪便pH值和消化率来反映),并通过使用氧化铬()、木质素()、不可消化酸性洗涤纤维()、不可消化中性洗涤纤维()和不可消化木质素()等内部和外部标记物来比较和验证干物质消化率的测量结果。九匹成年夸特马(六匹阉马,三匹母马)采用交叉设计,进行三个为期17天的饲养期(共51天),使用三种处理方式:对照组,不添加饲料添加剂();Smartpak(;马萨诸塞州普利茅斯);或Platinum Performance(;加利福尼亚州比尔敦)。SP和PP都含有一种 菌株,而SP还额外提供了甘露寡糖()和低聚果糖(),PP则提供了 在17天的时间段内,每天给马提供果园草干草和甜玉米谷物以及指定的处理剂,并在每个时间段的最后7天给马提供四块CR饼干,以提供8克/天的CR。在第15至17天收集全部粪便。将饲料和粪便样本干燥、研磨后送至ANALAB(伊利诺伊州富尔顿)进行养分分析。将饲料和粪便的重复样本放入装有瘤胃瘘管的奶牛体内,用于原位测定不可消化酸性洗涤纤维、不可消化中性洗涤纤维和不可消化木质素,以估计消化率。使用观测均值的均方根预测误差百分比()、一致性相关系数()和纳什-萨特克利夫效率方法评估估计的CR粪便产量、CR干物质摄入量和干物质消化率。标记物预测能力测试表明,不可消化酸性洗涤纤维的偏差最小,均方根预测误差百分比最小(4%),一致性相关系数最大(0.43),随机偏差量最大(离散误差 = 0.45)。补充PP会降低CR干物质消化率(<0.02)。Smartpak会提高粪便pH值(<0.09),但PP对粪便pH值没有影响。因此,SP对后肠健康有有益影响,据信这是由于甘露寡糖和低聚果糖所致。