Millican Allison A, Leatherwood Jessica L, Coverdale Josie A, Arnold Carolyn E, Bradbery Amanda N, Larson Connie K, Lamprecht Emily D, White Sarah H, Paulk Chad B, Welsh Thomas H, Wickersham Tryon A
Department of Animal Science, Texas A&M University, College Station, TX.
Department of Large Animal Clinical Sciences, Texas A&M University, College Station, TX.
Transl Anim Sci. 2020 Jan 17;4(2):txaa006. doi: 10.1093/tas/txaa006. eCollection 2020 Apr.
Sixteen weanling Quarter Horses (255 ± 22 kg) were utilized in a 56-d trial to evaluate the effects of trace mineral (TM) source on intra-articular inflammation following a single acute inflammatory insult. Horses were stratified by age, sex, and BW and then randomly assigned to dietary treatment: concentrate formulated with Zn, Mn, Cu, and Co as inorganic sources (CON; = 8) or complexed TMs (CTM; = 8). Added TM were formulated at iso-levels across treatments and intakes met or exceeded NRC requirements. Horses were offered 1.75% BW (as-fed) of treatment concentrate and 0.75% BW (as-fed) coastal Bermudagrass hay. Growth measurements were collected on days 0, 28, and 56, and plasma was collected biweekly for determination of Mn, Cu, Zn, and Co concentrations. On day 42, carpal joints were randomly assigned to receive injections of 0.5 ng lipopolysaccharide (LPS) or sterile lactated Ringer's solution (LRS; contralateral control). Synovial fluid was collected at preinjection hours (PIH) 0, and 6, 12, 24, 168, and 336 h post-injection and analyzed for TM concentration, prostaglandin E (PGE), carboxypeptide of type II collagen (CPII), collagenase cleavage neopeptide (C2C), and aggrecan chondroitin sulfate 846 epitope (CS846). Data were analyzed using the MIXED procedure of SAS. Results showed a TM source × LPS × h effect for synovial fluid Co, Cu, and Se ( < 0.05); concentrations of TM peaked at hour 6 and decreased to preinjection values by hour 168 in both CON and CTM-LPS knees. A delayed peak was observed at hour 12 for CTM-LRS. Peak synovial fluid Cu and Se concentrations were higher in LPS knees, and Co was highest in CTM-LPS. A TM source × h interaction was observed for Zn ( < 0.05); concentrations peaked at hour 6 in CON vs. hour 12 for CTM. An LPS × h interaction was observed for Mn ( < 0.01); synovial concentration peaked at hour 6 in LPS knees compared with hour 24 in LRS. Synovial PGE, C2C, CPII, and CS846 concentrations were greater with LPS ( ≤ 0.01), and C2C was greater ( < 0.01) in CTM compared with CON. Concentrations of CPII and PGE were unaffected by diet. A TM source × h × LPS interaction was observed for CS846 ( = 0.02). Concentrations of CS846 in CTM peaked at 12 h, whereas CON peaked at a lower concentration at 24 h ( < 0.05). Data indicate sufficient intake of a complexed TM source may support cartilage metabolism through increased aggrecan synthesis and type II collagen breakdown following an intra-articular LPS challenge in growing horses.
选用16匹断奶季马（体重255±22千克）进行一项为期56天的试验，以评估微量矿物质（TM）来源对单次急性炎症损伤后关节内炎症的影响。根据年龄、性别和体重对马匹进行分层，然后随机分配至日粮处理组：以锌、锰、铜和钴作为无机来源配制的精料（CON；n = 8）或复合微量矿物质（CTM；n = 8）。各处理组添加的微量矿物质按等水平配制，摄入量达到或超过NRC要求。给马匹提供1.75%体重（风干基础）的处理精料和0.75%体重（风干基础）的海滨百慕大草干草。在第0、28和56天收集生长指标数据，每两周采集血浆以测定锰、铜、锌和钴浓度。在第42天，随机选择腕关节注射0.5纳克脂多糖（LPS）或无菌乳酸林格氏液（LRS；对侧对照）。在注射前0小时（PIH）以及注射后6、12、24、168和336小时采集滑液，分析其中微量矿物质浓度、前列腺素E（PGE）、II型胶原羧基肽（CPII）、胶原酶裂解新肽（C2C）和聚集蛋白聚糖硫酸软骨素846表位（CS846）。使用SAS的MIXED程序分析数据。结果显示，滑液中钴、铜和硒存在微量矿物质来源×LPS×小时效应（P<0.05）；在CON和CTM-LPS膝关节中，微量矿物质浓度在6小时达到峰值，到168小时降至注射前水平。CTM-LRS在12小时出现延迟峰值。LPS膝关节中滑液铜和硒的峰值浓度较高，CTM-LPS中钴的浓度最高。锌存在微量矿物质来源×小时交互作用（P<0.05）；CON组在6小时达到峰值，而CTM组在12小时达到峰值。锰存在LPS×小时交互作用（P<0.01）；LPS膝关节中滑液浓度在6小时达到峰值，而LRS组在24小时达到峰值。LPS处理时滑液PGE、C2C、CPII和CS846浓度更高（P≤0.01），与CON相比，CTM组C2C浓度更高（P<0.01）。CPII和PGE浓度不受日粮影响。CS846存在微量矿物质来源×小时×LPS交互作用（P = 0.02）。CTM组CS846浓度在12小时达到峰值，而CON组在较低浓度的24小时达到峰值（P<0.05）。数据表明，充足摄入复合微量矿物质来源可能通过在生长马匹关节内LPS刺激后增加聚集蛋白聚糖合成和II型胶原分解来支持软骨代谢。
