Department of Experimental Biology, Jaén University, E-23071, Jaén, Spain.
Department of Animal and Plant Biology and Ecology, Jaén University, E-23071, Jaén, Spain.
Parasitol Res. 2020 Sep;119(9):2829-2835. doi: 10.1007/s00436-020-06824-y. Epub 2020 Jul 24.
Cephenemyia stimulator and Oestrus ovis are two important parasitic bot flies (Oestridae) species causing myiasis, with a potential negative impact on the welfare of the host. Using next-generation sequencing approach and bioinformatics tools, a large panel of possible microsatellites loci was obtained in both species. Primer pairs were designed for 15 selected microsatellite loci in C. stimulator and other 15 loci in O. ovis for PCR amplification. Loci amplification and analysis were performed in four populations of each species. The results demonstrated that all selected loci were polymorphic, with the number of alleles ranging from 2 to 6 per locus in C. stimulator and 3 to 13 per locus in O. ovis. This is the first time to describe these microsatellite loci for C. stimulator and O. ovis. These two sets of microsatellite markers could be further used for biogeographic and population genetics studies.
刺激库蠓和痒纹皮蝇是两种重要的寄生性狂蝇（皮蝇科）物种，会导致蝇蛆病，对宿主的福利有潜在的负面影响。本研究采用下一代测序方法和生物信息学工具，在这两个物种中获得了大量可能的微卫星基因座。针对刺激库蠓的 15 个微卫星基因座和痒纹皮蝇的 15 个微卫星基因座设计了引物对，用于 PCR 扩增。在每个物种的四个种群中进行了基因座扩增和分析。结果表明，所有选定的基因座均具有多态性，刺激库蠓每个基因座的等位基因数为 2-6 个，痒纹皮蝇每个基因座的等位基因数为 3-13 个。这是首次对刺激库蠓和痒纹皮蝇的这些微卫星基因座进行描述。这两套微卫星标记物可进一步用于生物地理和种群遗传学研究。
