Kinnear M W, Bariana H S, Sved J A, Frommer M
Fruit Fly Research Centre, School of Biological Sciences A12, University of Sydney, NSW, Australia.
Mol Ecol. 1998 Nov;7(11):1489-95. doi: 10.1046/j.1365-294x.1998.00480.x.
To obtain a set of microsatellite markers for the Queensland fruit fly Bactrocera tryoni, a genomic library was screened with a number of simple repeat oligonucleotide probes. Sequencing recovered 22 repeat loci. The microsatellite sequences were short, with repeat numbers ranging from five to 11. Of these, 16 polymerase chain reaction (PCR) primer sets yielded amplifiable products, which were tested on 53 flies from five widely separated sites. All loci showed polymorphism in the population sample, with the number of alleles ranging from two to 16. Several dinucleotide repeats showed alleles separated by single-base differences and multiple steps, suggesting a mutation process more complex than the stepwise mutation model.
为了获得昆士兰实蝇（Bactrocera tryoni）的一组微卫星标记，用一些简单重复寡核苷酸探针筛选了一个基因组文库。测序得到了22个重复位点。微卫星序列较短，重复次数从5到11不等。其中，16个聚合酶链反应（PCR）引物组产生了可扩增产物，并在来自五个相距甚远地点的53只果蝇上进行了测试。所有位点在群体样本中均表现出多态性，等位基因数量从2到16不等。几个二核苷酸重复序列显示等位基因之间存在单碱基差异和多步差异，这表明突变过程比逐步突变模型更为复杂。
