Karamian N A, Waters P F
J Pharm Sci. 1977 May;66(5):723-4. doi: 10.1002/jps.2600660529.
A spectrophotometric method is reported for assaying endotoxins over a wide concentration range, including low parts per billion levels. Five solution of endotoxins. Escherichia coli 0127:B8, Escherichia coli 055:B5, Salmonella abortus-equi, Salmonella enteritidis, and Shigella flexneri, were examined. Each exhibited an absorption maximum at 259 nm, but the absorptivities differed in each case. Thus, rigorous quantification requires that the identity of the endotoxin be known. The uv absorbance and the statistical data for the standard curves for three groups of standard solutions are presented. The absorbance for each group was linear with concentration within acceptable limits.
报道了一种分光光度法，可在很宽的浓度范围内测定内毒素，包括十亿分之几的低水平。对内毒素的五种溶液，即大肠杆菌0127:B8、大肠杆菌055:B5、马流产沙门氏菌、肠炎沙门氏菌和弗氏志贺氏菌进行了检测。每种溶液在259nm处均有最大吸收，但每种情况下的吸光率不同。因此，要进行精确的定量分析，需要知道内毒素的种类。给出了三组标准溶液的紫外吸光度和标准曲线的统计数据。在可接受的限度内，每组的吸光度与浓度呈线性关系。
