State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang, Jiangxi Province 330047, China; Institute of Food and Marine Bio-Resources, College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China.
State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang, Jiangxi Province 330047, China.
Int J Biol Macromol. 2020 Dec 1;164:826-835. doi: 10.1016/j.ijbiomac.2020.07.186. Epub 2020 Jul 21.
Glucomannans (GMs) from abundant natural resources have excellent processing properties and plentiful bioactivities. In current study, functional properties of GMs with different structural characteristics, including KGM from konjac, DOP from dendrobium, AGP, ASP-4N, ASP-6N, & ASP-8N from aloe were determined. Results suggested that molecular weights (M) of GMs were positively correlated with their water absorption capacity, fat absorption capacity, and viscosity, while ratio of mannose/glucose showed negative effect. Higher degree of acetylation (DA) mainly corresponded to higher values of solubility and ζ-potential. Then, effects of the six GMs on general health status, serum biochemicals, and intestinal SCFAs production in mice were evaluated in vivo. Analysis of general health status and levels of serum biochemicals revealed that mice with consecutive supplementation of GMs for 14 days performed normally compared with those in control group. Interestingly, the productions of SCFAs (mainly acetate and butyrate) in the cecal and colonic contents were significantly promoted. Generally, higher concentrations of SCFAs were produced when mice were treated with GMs having higher M, ratio of glucose, and DA. The current investigation suggested that both functional and intestinal fermentation property of GMs were jointly determined by the monosaccharide composition, molecular weight, and degree of acetylation.
葡甘露聚糖（GMs）来源于丰富的自然资源，具有优异的加工性能和丰富的生物活性。在本研究中，测定了具有不同结构特征的 GMs 的功能特性，包括魔芋中的 KGM、石斛中的 DOP、AGP、ASP-4N、ASP-6N 和芦荟中的 ASP-8N。结果表明，GMs 的分子量（M）与其吸水性、脂肪吸收能力和粘度呈正相关，而甘露糖/葡萄糖的比例则呈负相关。较高的乙酰化度（DA）主要对应于较高的溶解度和 ζ-电位。然后，在体内评估了这 6 种 GMs 对小鼠一般健康状况、血清生化指标和肠道 SCFAs 产生的影响。一般健康状况和血清生化指标分析表明，连续补充 GMs 14 天的小鼠与对照组相比表现正常。有趣的是，盲肠和结肠内容物中 SCFAs（主要是乙酸和丁酸）的产生明显增加。通常，当用具有较高 M、葡萄糖比例和 DA 的 GMs 处理小鼠时，SCFAs 的产量更高。本研究表明，GMs 的功能和肠道发酵特性是由单糖组成、分子量和乙酰化度共同决定的。
