Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
Gene. 2020 Oct 20;758:144968. doi: 10.1016/j.gene.2020.144968. Epub 2020 Jul 21.
The hair follicle is an excellent mini-system illustrating the mechanisms governing organogenesis and regeneration. Although the general mechanisms modulating skin and hair follicle development are widely studied in mouse and chicken models, the delicate network regulating skin and hair diversity remains largely unclear. Sheep is an additional model to address the various wool characteristics observed in nature. The coarse and fine wool sheep with diverse fibers were examined to show differences in the primary wool follicle size and skin thickness. The molecular dynamics in skin staged at the primary wool follicle induction between two sheep lines were investigated by RNA-sequencing analyses to generate 1994 differentially expressed genes revealing marker genes for epithelium (6 genes), dermal condensate (38 genes) and dermal fibroblast (58 genes) highly correlated with skin and wool follicle morphological differences. The DEGs were enriched in GO terms represented by epithelial cell migration and differentiation, regulation of hair follicle development and ectodermal placode formation, and KEGG pathways typified by WNT and Hedgehog signaling pathways governing the differences of skin structure. The qPCR detection of 9 genes confirmed the similar expression tendency with RNA-sequencing profiles. This comparative study of coarse and fine wool sheep skin reveals the presence of skin and wool follicle differences at primary wool follicle induction stage, and indicates the potential effectors (APCDD1, FGF20, DKK1, IGFBP3 and SFRP4) regulating the skin compartments during the early morphogenesis of primary wool follicles to shape the variable wool fiber thickness in later developmental stages.
毛囊是一个极好的小型系统，阐明了器官发生和再生的机制。尽管在小鼠和鸡模型中广泛研究了调节皮肤和毛囊发育的一般机制，但调节皮肤和毛发多样性的精细网络在很大程度上仍不清楚。绵羊是另一种模型，可以解决自然界中观察到的各种羊毛特征。对具有不同纤维的粗毛和细毛绵羊进行了检查，以显示初级羊毛毛囊大小和皮肤厚度的差异。通过 RNA 测序分析研究了两个绵羊系之间初级羊毛毛囊诱导过程中的皮肤阶段的分子动力学，生成了 1994 个差异表达基因，揭示了与皮肤和毛囊形态差异高度相关的上皮（6 个基因）、真皮凝聚物（38 个基因）和真皮成纤维细胞（58 个基因）的标记基因。差异表达基因在 GO 术语中富集，代表上皮细胞迁移和分化、毛囊发育和外胚层基板形成的调节，以及 WNT 和 Hedgehog 信号通路等 KEGG 通路，这些通路控制着皮肤结构的差异。9 个基因的 qPCR 检测证实了与 RNA 测序图谱相似的表达趋势。对粗毛和细毛绵羊皮肤的比较研究揭示了初级羊毛毛囊诱导阶段皮肤和羊毛毛囊的差异，并表明了潜在的效应物（APCDD1、FGF20、DKK1、IGFBP3 和 SFRP4）在初级羊毛毛囊早期形态发生过程中调节皮肤区室，以在后期发育阶段塑造不同的羊毛纤维厚度。
