Norup Pernille Badsberg, Egeberg Palme Dorte L, Petersen Morten R, Main Katharina M, Almstrup Kristian
Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen, Denmark.
International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen, Denmark.
J Clin Med. 2020 Jul 22;9(8):2327. doi: 10.3390/jcm9082327.
The acrosome of the spermatozoa is required for fertilization and in the raw ejaculate the percentage of viable acrosome-intact spermatozoa, the acrosomal status, is higher among men with good semen quality. Here we investigated if the acrosomal status of the processed semen preparations used at a fertility clinic can also be informative and whether it is associated with fecundity. The acrosomal status was measured by image cytometry on purified semen samples from couples during in vitro fertilization (IVF) ( = 99) and intracytoplasmic sperm injection (ICSI) ( = 107) treatment. Purified frozen-thawed donor samples were also analyzed ( = 199). In purified semen preparations the acrosomal status was significantly higher among sperm donors ( = 5.3 × 10) and men from IVF couples ( = 2.2 × 10) when compared to men from ICSI couples. A significant difference was also found between female, male and mixed factor infertility ( = 0.003). No association with lifestyle factors was found. In frozen-thawed donor samples, a significant positive (r = 0.16, = 0.025) association with the number of pregnancies per sold straw was observed together with an area under the curve of 75.3%, when comparing the top and bottom deciles. Our results indicate that the acrosomal status may be a valuable parameter for personalizing fertility treatments and might be a good predictor of pregnancy success among normozoospermic men.
精子的顶体对于受精是必需的，在原始射精中，精液质量良好的男性中，具有活力且顶体完整的精子百分比（顶体状态）更高。在此，我们研究了生育诊所使用的处理后精液制剂的顶体状态是否也能提供信息，以及它是否与生育能力相关。通过图像细胞术对体外受精（IVF）（n = 99）和卵胞浆内单精子注射（ICSI）（n = 107）治疗期间夫妇的纯化精液样本测量顶体状态。还分析了纯化的冻融供体样本（n = 199）。与ICSI夫妇的男性相比，在纯化精液制剂中，精子供体（n = 5.3×10）和IVF夫妇的男性（n = 2.2×10）的顶体状态显著更高。在女性、男性和混合因素不孕症之间也发现了显著差异（P = 0.003）。未发现与生活方式因素相关。在冻融供体样本中，比较最高和最低十分位数时，观察到与每售出一支细管的妊娠次数存在显著正相关（r = 0.16，P = 0.025），曲线下面积为75.3%。我们的结果表明，顶体状态可能是个性化生育治疗的一个有价值的参数，并且可能是正常精子症男性妊娠成功的良好预测指标。
