Albani Elena, Castellano Stefano, Gurrieri Bruna, Arruzzolo Luisa, Negri Luciano, Borroni Elena M, Levi-Setti Paolo E
Division of Gynecology and Reproductive Medicine, Department of Gynecology, Humanitas Fertility Center, Humanitas Research Hospital, 20089 Rozzano (Milan), Italy.
Humanitas Clinical and Research Center, 20089 Rozzano (Milan), Italy.
Aging (Albany NY). 2019 May 14;11(9):2749-2761. doi: 10.18632/aging.101946.
The main goal of semen processing in Assisted Reproductive Techniques (ART) is to select sperm with good viability and, at the same time, remove Reactive Oxygen Species (ROS) sources (such as leukocytes) and reduce the percentage of morphologically abnormal sperm for fertility treatment. We performed a comparative analysis on sperm DNA fragmentation after Density Gradient Centrifugation (DGC) using products sold by two competing companies. Our results showed comparable DNA Fragmentation Index (DFI) after treatment with both DGC products. However, in both cases, a comparable number of samples do not benefit from the treatment. Interestingly, increasing evidences indicated that male age has a negative impact on sperm DNA fragmentation, but the mechanisms underlying age-dependent patterns of sperm decline have not yet been fully understood. Thus, we performed a comparative analysis of DFI before and after treatment with DGC products in age-stratified sample populations. Our results showed a worsening of the baseline DFI in the eldest group and the benefits of DGC on sperm DNA were compromised. In conclusion, our work consolidates the current evidences suggesting that both paternal and maternal aging, critically affects reproductive success.
辅助生殖技术（ART）中精液处理的主要目标是选择活力良好的精子，同时去除活性氧（ROS）来源（如白细胞），并降低形态异常精子的比例以进行生育治疗。我们使用两家竞争公司销售的产品，对密度梯度离心（DGC）后的精子DNA片段化进行了比较分析。我们的结果显示，使用两种DGC产品处理后，DNA片段化指数（DFI）相当。然而，在这两种情况下，相当数量的样本并未从该处理中受益。有趣的是，越来越多的证据表明男性年龄对精子DNA片段化有负面影响，但精子随年龄下降模式的潜在机制尚未完全了解。因此，我们在年龄分层的样本群体中对DGC产品处理前后的DFI进行了比较分析。我们的结果显示，最年长组的基线DFI恶化，DGC对精子DNA的益处受到损害。总之，我们的工作巩固了目前的证据，表明父母双方的年龄都会严重影响生殖成功率。
