Department of Social Medicine and Public Health, Medical University of Warsaw, Oczki 3, 02-007 Warsaw, Poland.
Int J Environ Res Public Health. 2020 Jul 16;17(14):5131. doi: 10.3390/ijerph17145131.
Hospital-acquired infections (HAIs) remain a common problem, which suggests that standard decontamination procedures are insufficient. Thus, new methods of decontamination are needed in hospitals.
We assessed the effectiveness of a no-touch automated disinfection (NTD) system in the decontamination of 50 surfaces in 10 hospital rooms. Contamination of surfaces was assessed with a microbiological assay and an ATP bioluminescence assay. Unacceptable contamination was defined as > 100 colony forming units/100 cm in the microbiological assay, and as ≥ 250 relative light units in the ATP assay.
When measured with the microbiological assay, 11 of 50 surfaces had unacceptable contamination before NTD, and none of the surfaces had unacceptable contamination after NTD ( < 0.001). On the ATP bioluminescence assay, NTD decreased the number of surfaces with unacceptable contamination from 28 to 13, but this effect was non-significant ( = 0.176). On the microbiological assay taken before NTD, the greatest contamination exceeded the acceptable level by more than 11-fold (lamp holder, 1150 CFU/100 cm). On the ATP bioluminescence assay taken before NTD, the greatest contamination exceeded the acceptable level by more than 43-fold (Ambu bag, 10,874 RLU).
NTD effectively reduced microbiological contamination in all hospital rooms. However, when measured with the ATP bioluminescence assay, the reduction of contamination was not significant.
医院获得性感染（HAIs）仍然是一个常见问题，这表明标准的消毒程序还不够。因此，医院需要新的消毒方法。
我们评估了无接触自动化消毒（NTD）系统对 10 个病房 50 个表面进行消毒的效果。通过微生物检测和 ATP 生物发光检测评估表面污染情况。微生物检测中> 100 个菌落形成单位/100 cm 定义为不可接受的污染，ATP 检测中≥ 250 相对光单位定义为不可接受的污染。
使用微生物检测时，NTD 前 50 个表面中有 11 个表面存在不可接受的污染，NTD 后所有表面均无不可接受的污染（< 0.001）。使用 ATP 生物发光检测时，NTD 将不可接受污染的表面数量从 28 个减少到 13 个，但无统计学意义（= 0.176）。在 NTD 前进行的微生物检测中，污染最严重的表面超过可接受水平超过 11 倍（灯座，1150 CFU/100 cm）。在 NTD 前进行的 ATP 生物发光检测中，污染最严重的表面超过可接受水平超过 43 倍（复苏袋，10874 RLU）。
NTD 有效地降低了所有病房的微生物污染。然而，使用 ATP 生物发光检测时，污染的减少并不显著。
