Liu Zhiqian, Ezernieks Vilnis, Reddy Priyanka, Elkins Aaron, Krill Christian, Murphy Kieran, Rochfort Simone, Spangenberg German
Agriculture Victoria Research, AgriBio, 5 Ring Road, Bundoora, VIC 3083, Australia.
School of Applied Systems Biology, La Trobe University, Bundoora, VIC 3083, Australia.
Metabolites. 2020 Jul 17;10(7):294. doi: 10.3390/metabo10070294.
Volatile phenols (VPs) derived from smoke-exposed grapes are known to confer a smoky flavor to wine. Current methods for determination of VPs in grape berries either involve complex sample purification/derivatization steps or employ two analytical platforms for free and bound VP fractions. We report here a simple gas chromatography-tandem mass spectrometry (GC-MS/MS) method for quantification of both free and bound VPs in grapes, based on optimized (1) GC-MS/MS parameters, (2) an analyte extraction procedure, and (3) phenol glycoside hydrolysis conditions. Requiring neither sample cleanup nor a derivatization step, this method is sensitive (LOD ≤ 1 ng/g berries) and reproducible (RSD < 12% for repeated analyses) and is expected to significantly reduce the sample turnover time for smoke taint detection in vineyards.
已知暴露于烟雾中的葡萄产生的挥发性酚类物质（VPs）能赋予葡萄酒烟熏风味。目前用于测定葡萄浆果中VPs的方法要么涉及复杂的样品净化/衍生步骤，要么采用两个分析平台分别测定游离态和结合态VP组分。我们在此报告一种简单的气相色谱-串联质谱法（GC-MS/MS），用于定量葡萄中的游离态和结合态VPs，该方法基于优化的（1）GC-MS/MS参数、（2）分析物提取程序和（3）酚糖苷水解条件。该方法既不需要样品净化也不需要衍生步骤，具有高灵敏度（检测限≤1 ng/g浆果）和高重现性（重复分析的相对标准偏差<12%），有望显著缩短葡萄园烟雾污染检测的样品周转时间。
