J Integr Plant Biol. 2014 Sep;56(9):816-25. doi: 10.1111/jipb.12181.
Metabolite analysis or metabolomics is an important component of systems biology in the post-genomic era. Although separate liquid chromatography (LC) methods for quantification of the major classes of polar metabolites of plants have been available for decades, a single method that enables simultaneous determination of hundreds of polar metabolites is possible only with gas chromatography–mass spectrometry (GC–MS) techniques. The rapid expansion of new LC stationary phases in the market and the ready access of mass spectrometry in many laboratories provides an excellent opportunity for developing LC–MS based methods for multi-target quantification of polar metabolites. Although various LC–MS methods have been developed over the last 10 years with the aim to quantify one or more classes of polar compounds in different matrices, currently there is no consensus LC–MS method that is widely used in plant metabolomics studies. The most promising methods applicable to plant metabolite analysis will be reviewed in this paper and the major problems encountered highlighted. The aim of this review is to provide plant scientists, with limited to moderate experience in analytical chemistry, with up-to-date and simplified information regarding the current status of polar metabolite analysis using LC–MS techniques.
代谢物分析或代谢组学是后基因组时代系统生物学的一个重要组成部分。尽管几十年来一直有用于定量植物中主要类极性代谢物的单独液相色谱 (LC) 方法，但只有使用气相色谱-质谱 (GC-MS) 技术才有可能同时测定数百种极性代谢物。市场上新型 LC 固定相的快速扩展以及许多实验室中质谱的便捷获取，为开发基于 LC-MS 的用于多靶点定量分析极性代谢物的方法提供了极好的机会。尽管在过去 10 年中已经开发了各种 LC-MS 方法，旨在定量不同基质中一种或多种类别的极性化合物，但目前在植物代谢组学研究中没有广泛使用的共识 LC-MS 方法。本文将综述适用于植物代谢物分析的最有前途的方法，并强调遇到的主要问题。本文的目的是为具有有限或中等分析化学经验的植物科学家提供有关使用 LC-MS 技术进行极性代谢物分析的最新简化信息。
