[漱口水对白色念珠菌和粘红酵母的抗真菌活性：一项体外研究]

[Antifungal activity of mouthwashes against Candida albicans and Rhodotorula mucilaginosa: An in vitro study].

作者信息

Handschuh Briones Rodrigo Alejandro, Silva Arcos Evelyn Nicole, Urrutia Milton, Godoy-Martínez Patricio

机构信息

Escuela de Odontología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile.

Universidad de Antofagasta, Antofagasta, Chile.

出版信息

Rev Iberoam Micol. 2020 Apr-Jun;37(2):47-52. doi: 10.1016/j.riam.2019.10.006. Epub 2020 Jul 21.

DOI:10.1016/j.riam.2019.10.006

PMID:32709501

Abstract

BACKGROUND

Candida albicans and Rhodotorula mucilaginosa are yeasts of clinical importance in the oral cavity. In immunocompromised patients they can cause some pathologies that must be controlled with antimicrobials.

AIMS

To evaluate and compare the antimicrobial efficacy of commercially available mouthrinses against strains of C. albicans and R. mucilaginosa.

METHODS

The six mouthwashes studied in vitro were formulated (alone or in combination) with chlorhexidine (CHX) 0.12%, CHX 0.1%, CHX 0.05%, cetylpyridinium chloride (CPC) 0.075%, CPC 0.05%, and essential oils. Ten C. albicans and R. mucilaginosa isolates each were studied. The agar diffusion method (Mueller Hinton II), with incubation at 32°C was used to evaluate the antifungal activity.

RESULTS

The results of this study indicate that mouthwashes with CHX 0.1%, CHX 0.12%, CHX 0.05% + CPC 0.05%, CHX 0.12% + CPC 0.05% and CPC 0.075% have an antifungal effect against C. albicans and R. mucilaginosa. CHX 0.1% led to the broadest inhibition zone for C. albicans and R. mucilaginosa (25.65±2.39mm and 40.05±3.31mm). Essential oils did not show any antifungal activity. Statistical analysis showed no statistical difference between mouth rinses CHX 0.1%, CHX 0.12% and CHX 0.12% + CPC 0.05% (p=0.0001) against C. albicans and R. mucilaginosa.

CONCLUSIONS

Mouthwashes with CHX showed higher antifungal activity against C. albicans and R. mucilaginosa than other mouthwashes studied.

摘要

背景

白色念珠菌和粘红酵母是口腔中具有临床重要性的酵母。在免疫功能低下的患者中，它们可引发一些必须用抗菌药物控制的病症。

目的

评估并比较市售漱口水对白色念珠菌和粘红酵母菌株的抗菌效果。

方法

体外研究的六种漱口水单独或联合使用0.12%洗必泰（CHX）、0.1% CHX、0.05% CHX、0.075%西吡氯铵（CPC）、0.05% CPC以及精油配制而成。每种分别研究了10株白色念珠菌和粘红酵母分离株。采用在32°C孵育的琼脂扩散法（穆勒-欣顿II）评估抗真菌活性。

结果

本研究结果表明，含0.1% CHX、0.12% CHX、0.05% CHX + 0.05% CPC、0.12% CHX + 0.05% CPC和0.075% CPC的漱口水对白色念珠菌和粘红酵母具有抗真菌作用。0.1% CHX对白色念珠菌和粘红酵母产生的抑菌圈最宽（分别为25.65±2.39毫米和40.05±3.31毫米）。精油未显示出任何抗真菌活性。统计分析表明，含0.1% CHX、0.12% CHX和0.12% CHX + 0.05% CPC的漱口水对白色念珠菌和粘红酵母无统计学差异（p = 0.0001）。