Cuenca-León Katherine, Sarmiento-Ordóñez Jéssica María, Pacheco-Quito Edisson-Mauricio, Benavides-Túlcan Samantha Miriam, Castro Nicole Alejandra, Zarzuelo-Castañeda Aránzazu
Unidad Académica de Salud y Bienestar, Carrera de Odontología, Universidad Católica de Cuenca, Cuenca, Ecuador.
Grupo de Investigación Innovación y Desarrollo Farmacéutico en Odontología, Jefatura de Investigación e Innovación, Universidad Católica de Cuenca, Cuenca, Ecuador.
J Exp Pharmacol. 2025 Jul 3;17:455-466. doi: 10.2147/JEP.S521028. eCollection 2025.
In this study, the efficacy of three essential oil extraction techniques steam entrainment, Soxhlet extraction, and supercritical fluid extraction (SCF) was evaluated.
Determine the most effective method for obtaining bioactive compounds from , and , plant species recognized for their antimicrobial and anti-inflammatory properties with potential applications in dentistry. The selected plants, identified based on scientific evidence, were certified and lyophilized prior to extraction. The efficiency of each technique was assessed in terms of essential oil yield, purity, and biological activity.
The plant species were selected for their therapeutic properties based on scientific reports, certified, and lyophilized before proceeding with the different extraction methods. The efficacy of each technique was compared in terms of the purity and yield of the essential oils obtained. The statistical analysis was performed using SPSS version 25.
It was shown that FSC extraction is the method that allows obtaining essential oils with the highest purity and yield, followed by steam entrainment, with the Soxhlet method being the least efficient. However, in the microbiological analysis, the essential oil obtained by steam entrainment showed a greater inhibitory effect against ATCC 90028.
In this study, the FSC and steam entrainment techniques are the most suitable for obtaining essential oils for therapeutic applications in dentistry, since they allow better preservation of the bioactive components and reduce the environmental impact. These results position FSC and vapor entrainment extraction as promising options for the development of therapeutic products in dentistry.
在本研究中，评估了三种精油提取技术——蒸汽夹带法、索氏提取法和超临界流体萃取法（SCF）的效果。
确定从具有抗菌和抗炎特性且在牙科有潜在应用价值的、和植物物种中获取生物活性化合物的最有效方法。所选植物根据科学证据进行鉴定，在提取前经过认证并冻干。从精油产量、纯度和生物活性方面评估每种技术的效率。
根据科学报告选择具有治疗特性的植物物种，在进行不同提取方法之前进行认证并冻干。比较每种技术在所得精油纯度和产量方面的效果。使用SPSS 25版进行统计分析。
结果表明，超临界流体萃取法是能够获得纯度和产量最高的精油的方法，其次是蒸汽夹带法，索氏提取法效率最低。然而，在微生物分析中，通过蒸汽夹带法获得的精油对ATCC 90028显示出更大的抑制作用。
在本研究中，超临界流体萃取法和蒸汽夹带技术最适合用于获取牙科治疗应用的精油，因为它们能更好地保存生物活性成分并减少环境影响。这些结果表明超临界流体萃取法和蒸汽夹带萃取法是牙科治疗产品开发的有前景的选择。
