Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China.
Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China; Zhejiang Huacai Testing Technology Co., Ltd., Shaoxing, China.
Food Chem. 2021 Jan 1;334:127558. doi: 10.1016/j.foodchem.2020.127558. Epub 2020 Jul 17.
Shellfishes contain plasmalogens correlating to the functions of brain, heart, etc. Herein, a mild acid hydrolysis and hydrophilic interaction chromatography (HILIC) tandem mass spectrometry method was developed for analyzing plasmalogens in six shellfish species. A total of 19 plasmalogen molecular species were successfully identified, including nine phosphatidylcholine plasmalogen (plasPC), seven phosphatidylethanolamine plasmalogen (plasPE), and three phosphatidylserine plasmalogen (plasPS). The quantitative results indicated that mussel (32 μg·mg) possessed the highest content of plasmalogens, followed by oyster (21 μg·mg) and razor clam (15 μg·mg). The statistic models showed that the plasPE P-18:0/20:5 (m/z 748), plasPE P-16:0/22:2 & P-18:0/20:2 (m/z 754) and plasPS were the most contributing difference between shellfishes. The results indicated that this method was sensitive and precise to determine plasmalogens in shellfish, and mussel was demonstrated to be a good choice for the large-scale preparation of plasmalogens.
贝类含有与脑、心等功能相关的类脂体。本研究建立了一种温和酸水解-亲水作用色谱（HILIC）串联质谱法，用于分析 6 种贝类中的类脂体。成功鉴定了 19 种类脂体分子种类，包括 9 种磷脂酰胆碱类脂体（plasPC）、7 种磷脂酰乙醇胺类脂体（plasPE）和 3 种磷脂酰丝氨酸类脂体（plasPS）。定量结果表明，贻贝类（32μg·mg）的类脂体含量最高，其次是牡蛎（21μg·mg）和文蛤（15μg·mg）。统计模型表明，类脂体 PE P-18:0/20:5（m/z 748）、PE P-16:0/22:2 & P-18:0/20:2（m/z 754）和 PS 是贝类之间差异最显著的物质。结果表明，该方法灵敏、准确，适用于贝类中类脂体的测定，贻贝类是大规模制备类脂体的良好选择。
