Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
Food Chem. 2021 Jan 1;334:127607. doi: 10.1016/j.foodchem.2020.127607. Epub 2020 Jul 19.
We present a simple and sensitive method for the determination of patulin at µg·kg level in apple-based products. Our method relies on the application of an in-line molecularly imprinted polymer solid-phase extraction microcartridge in capillary electrophoresis coupled with mass spectrometry. Capillary zone electrophoresis method has been developed and parameters affecting the in-line process have been carefully optimized. Validation parameters were assessed for patulin, giving LOQ of 1 µg·kg and linearity range 1-100 µg·kg with R ≥ 0.997. The LOQ was below the maximum content of patulin requested by the European Union in this type of products. The precision of the peak area and the migration time were less than 14.9 and 1.6%, respectively. Patulin has been analyzed in the presence of 5-hydroxymethylfurfural, which is the main interference in this kind of matrix. The method was applied to assay patulin content in various apple-based products.
我们提出了一种简单灵敏的方法，用于在基于苹果的产品中测定微克/千克水平的棒曲霉素。我们的方法依赖于在线分子印迹聚合物固相萃取微柱在毛细管电泳与质谱联用中的应用。已经开发了毛细管区带电泳方法，并仔细优化了影响在线过程的参数。对棒曲霉素进行了验证参数评估，得到了 1μg·kg 的 LOQ 和 1-100μg·kg 的线性范围，R≥0.997。LOQ 低于欧盟对此类产品中棒曲霉素最大含量的要求。峰面积和迁移时间的精密度分别小于 14.9%和 1.6%。在 5-羟甲基糠醛存在下分析了棒曲霉素，5-羟甲基糠醛是这种基质中的主要干扰物。该方法已应用于各种基于苹果的产品中棒曲霉素含量的测定。
