纳米氧化铈通过实时荧光定量聚合酶链反应促进肝癌细胞增殖并调节凋亡相关基因 Bcl-2 和 Bax 的 mRNA 表达。

Nano-Cerium Oxide Promotes Proliferation of Hepatoma Cells and Regulates mRNA Expression of Apoptosis-Related Genes Bcl-2 and Bax, as Detected Through Real-Time Fluorescent Quantitative Polymerase Chain Reaction.

机构信息

Intervention Therapy Department, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, PR China.

Nursing Department, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, PR China.

出版信息

J Nanosci Nanotechnol. 2020 Dec 1;20(12):7457-7463. doi: 10.1166/jnn.2020.18718.

DOI:10.1166/jnn.2020.18718

PMID:32711615

Abstract

In this study, we aim to investigate the effects of nano-cerium oxide (CNPs) on the proliferation of hepatoma cells and expression of the Bcl-2 and Bax mRNAs. Huh7, 7721 and HepG2 liver cancer cells were cultured and treated with CNPs at concentrations of 0.005, 0.01, 0.05, 0.1, and 1 g/mL. The effect of the CNPs on the proliferation of hepatoma cells were detected by an electrochemical method. The expressions of the Bcl-2 and Bax mRNAs were evaluated by qRT-PCR. Additionally, the effect of CNPs on the cell cycle was investigated by flow cytometry. Low concentrations of CNPs have a proliferative effect on hepatoma cells. qRT-PCR showed that CNPs could inhibit the apoptosis of hepatoma cells. Flow cytometry showed that CNPs had no effect on the hepatocellular carcinoma cell cycle. Low concentrations of CNPs have a proliferative effect on hepatoma cells.

摘要

在这项研究中，我们旨在探讨纳米氧化铈（CNPs）对肝癌细胞增殖和 Bcl-2 和 Bax mRNA 表达的影响。培养 Huh7、7721 和 HepG2 肝癌细胞，并分别用浓度为 0.005、0.01、0.05、0.1 和 1 g/ml 的 CNPs 处理。采用电化学方法检测 CNPs 对肝癌细胞增殖的影响。通过 qRT-PCR 评估 Bcl-2 和 Bax mRNA 的表达。此外，通过流式细胞术研究了 CNPs 对细胞周期的影响。低浓度的 CNPs 对肝癌细胞具有增殖作用。qRT-PCR 显示 CNPs 可以抑制肝癌细胞的凋亡。流式细胞术显示 CNPs 对肝癌细胞周期没有影响。低浓度的 CNPs 对肝癌细胞具有增殖作用。