Jiangsu Key Laboratory for Biomaterials and Devices, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, P. R. China.
J Nanosci Nanotechnol. 2020 Dec 1;20(12):7583-7592. doi: 10.1166/jnn.2020.18622.
Phospholipid liposomes are a promising drug delivery system. Catechin, a hydrophilic drug, was used to prepare catechin liposomes through a modified rapid expansion of supercritical solution (RESS) process in this study. The influences of operation parameters (i.e., temperature, pressure, and mass ratio of liposomal materials to catechin) on the properties of the prepared liposomes were determined using the single-factor analysis. The process was further optimized by response surface methodology (RSM) based on the Box-Behnken design (BBD). The encapsulation efficiency (EE) values can be adequately predicted using the obtained equation. The maximum EE value can reach 61.36±0.68% under the optimal parameters (i.e., the expansion temperature, pressure, and p/c mass ratio were 56.34 °C, 19.99 MPa, and 5.99, respectively). The prepared liposomes can effectively protect and stabilize the loaded catechin effectively. In addition, the release study showed the slow and sustained release behavior of the catechin liposomes.
脂质体是一种很有前途的药物传递系统。儿茶素是一种亲水性药物，本研究通过改良的快速扩张超临界溶液（RESS）工艺将儿茶素制备成脂质体。通过单因素分析确定了操作参数（即温度、压力和脂质体材料与儿茶素的质量比）对所制备脂质体性质的影响。该过程进一步通过基于 Box-Behnken 设计（BBD）的响应面法（RSM）进行了优化。使用获得的方程可以充分预测包封效率（EE）值。在最佳参数（即膨胀温度、压力和 p/c 质量比分别为 56.34°C、19.99 MPa 和 5.99）下，最大 EE 值可达 61.36±0.68%。所制备的脂质体可以有效地保护和稳定负载的儿茶素。此外，释放研究表明儿茶素脂质体具有缓慢和持续的释放行为。
