a College of Food Science , South China Agricultural University , Guangzhou , Guangdong , P. R. China.
J Microencapsul. 2017 Nov;34(7):687-698. doi: 10.1080/02652048.2017.1376001. Epub 2017 Sep 13.
Melatonin-loaded liposomes (MLL) were successfully prepared using rapid expansion of supercritical solution technology. The effects of supercritical pressure on encapsulation efficiency (EE) and average particle size were then analysed. Meanwhile, temperature, formation time and ethanol concentration in the products were studied and optimised based on the response surface methodology (RSM). An in vitro simulated digestion model was also established to evaluate the release performance of MLL. The results showed that 140 bar was the best pressure for maximising the EE value using RSM optimisation, reaching up to 82.2%. MLL characterisations were performed using analytic techniques including infrared spectroscopy, transmission electron microscopy, a laser scattering particle size analyser and gas chromatograph-mass spectrometer. The size distribution was uniform, with an average diameter of 66 nm. Stability tests proved that MLL maintained good preservation duration, and residual solvent experiments indicated that only 1.03% (mass ratio) of ethanol remained in the products. Simulated release experiments indicated the slow release feature in early digestive stages and more thorough characteristics in later stages of simulated digestion.
采用超临界溶液快速扩展技术成功制备了载有褪黑素的脂质体（MLL）。然后分析了超临界压力对包封效率（EE）和平均粒径的影响。同时，基于响应面法（RSM）研究并优化了产品中的温度、形成时间和乙醇浓度。还建立了体外模拟消化模型来评估 MLL 的释放性能。结果表明，使用 RSM 优化，140 bar 是获得最大 EE 值的最佳压力，达到 82.2%。采用红外光谱、透射电子显微镜、激光散射粒度分析仪和气相色谱-质谱联用仪等分析技术对 MLL 进行了表征。粒径分布均匀，平均直径为 66nm。稳定性测试证明 MLL 保持了良好的保存期限，残留溶剂实验表明产品中仅残留 1.03%（质量比）的乙醇。模拟释放实验表明，在早期消化阶段具有缓慢释放的特点，在模拟消化的后期阶段具有更彻底的特点。
