Anhui Province Key Laboratory for Conservation and Exploitation of Biological Resource, College of Life Sciences, Anhui Normal University, Wuhu 241000, Anhui, China.
Anhui Province Key Laboratory for Conservation and Exploitation of Biological Resource, College of Life Sciences, Anhui Normal University, Wuhu 241000, Anhui, China.
Comp Biochem Physiol B Biochem Mol Biol. 2020 Oct-Nov;248-249:110482. doi: 10.1016/j.cbpb.2020.110482. Epub 2020 Jul 24.
In some countries, snakes are important protein sources in human diets, and their economic value depends predominantly on their muscle production, including in the king ratsnake (Elaphe carinata). Muscle growth in the king ratsnake clearly differs among individuals. To date, few potential molecular mechanisms underlying these differences in muscle growth and development have been reported. Here, we integrated mRNA and miRNA expression profiles to screen for genes, pathways, and predicted miRNA-mRNA networks associated with muscle growth and development in fast-growing and slow-growing King ratsnakes. Six hundred eight differentially expressed genes (DEGs) were identified, 48 of which were associated with muscle growth. The 37 genes upregulated in fast-growing individuals (FGIs) may be related to the promotion of muscle growth, whereas the 11 upregulated genes in slow-growing individuals (SGIs) may be related to the inhibition of muscle growth. Seven DEGs were enriched in the PI3K-AKT-MTOR signaling pathway, which appears to promote muscle growth in FGIs. Eleven DEGs were enriched in the ubiquitin-proteasome pathway, which appears to inhibit muscle growth in SGIs. It may interpret why muscle growth differences. Furthermore, 698 miRNA were identified, including 125 novel miRNAs. 63 differentially expressed miRNA (DEMs) were screened, and 950 negative miRNA-mRNA interactions with the 63 DEMs and 608 DEGs were predicted. The miRNA-targeted genes were enriched in pathways related to muscle growth, protein synthesis, and protein degradation. Therefore, in addition to the identified DEGs, miRNAs may play important roles in the differential regulation of muscle growth in FGIs and SGIs of the king ratsnake.
在一些国家，蛇是人类饮食中重要的蛋白质来源，其经济价值主要取决于肌肉产量，包括王锦蛇（Elaphe carinata）在内。王锦蛇个体间肌肉生长差异明显。迄今为止，关于肌肉生长和发育差异的潜在分子机制鲜有报道。在这里，我们整合了 mRNA 和 miRNA 表达谱，筛选与快速生长和慢速生长王锦蛇肌肉生长和发育相关的基因、途径和预测的 miRNA-mRNA 网络。鉴定了 608 个差异表达基因（DEGs），其中 48 个与肌肉生长有关。在快速生长个体（FGIs）中上调的 37 个基因可能与促进肌肉生长有关，而在慢速生长个体（SGIs）中上调的 11 个基因可能与抑制肌肉生长有关。七个 DEGs 富集在 PI3K-AKT-MTOR 信号通路中，该通路似乎促进了 FGIs 的肌肉生长。11 个 DEGs 富集在泛素蛋白酶体途径中，该途径似乎抑制了 SGIs 的肌肉生长。这可能解释了肌肉生长差异的原因。此外，鉴定了 698 个 miRNA，包括 125 个新 miRNA。筛选出 63 个差异表达 miRNA（DEMs），并预测了 63 个 DEMs 和 608 个 DEGs 与 950 个负 miRNA-mRNA 相互作用。miRNA 靶向基因富集在与肌肉生长、蛋白质合成和蛋白质降解相关的途径中。因此，除了鉴定出的 DEGs 外，miRNAs 可能在 FGIs 和 SGIs 的肌肉生长差异调节中发挥重要作用。
